Align aconitate DELTA-isomerase (EC 5.3.3.7) (characterized)
to candidate 7026695 Shewana3_3828 methylitaconate delta2-delta3-isomerase (RefSeq)
Query= BRENDA::Q8EJW4 (397 letters) >FitnessBrowser__ANA3:7026695 Length = 397 Score = 729 bits (1881), Expect = 0.0 Identities = 366/395 (92%), Positives = 378/395 (95%) Query: 1 MSNKLFPPQIKVAATYMRGGTSKGVFFRLQDLPEAAQVPGPARDALLLRVIGSPDPYAKQ 60 MS FPPQIKVAATYMRGGTSKGVFFRLQDLPEAAQVPGPARDALLLRVIGSPDPY KQ Sbjct: 1 MSKNPFPPQIKVAATYMRGGTSKGVFFRLQDLPEAAQVPGPARDALLLRVIGSPDPYGKQ 60 Query: 61 IDGMGGATSSTSKTVILSHSSKANHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAIS 120 IDGMGGATSSTSKTVILS S++A+HDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAIS Sbjct: 61 IDGMGGATSSTSKTVILSKSTQADHDVDYLFGQVSIDKPFVDWSGNCGNLTAAVGAFAIS 120 Query: 121 NGLIDAARIPRNGVCTVRIWQANIGKTIIAHVPITDGAVQETGDFELDGVTFPAAEVQIE 180 NGLIDA RIP+NG+CTVRIWQANI KTIIAHVPIT+G VQETGDFELDGVTFPAAEVQIE Sbjct: 121 NGLIDAERIPQNGICTVRIWQANIAKTIIAHVPITNGEVQETGDFELDGVTFPAAEVQIE 180 Query: 181 FMNPAADDDGEGGCMFPTGNLVDVLEVPGIGRFNATMINAGIPTIFINAEDLGYTGTELQ 240 FMNPAAD+DG+ G MFPTGNLVDVLEVPGIG FNATMINAGIPTIFINAEDLGYTGTELQ Sbjct: 181 FMNPAADEDGDSGSMFPTGNLVDVLEVPGIGSFNATMINAGIPTIFINAEDLGYTGTELQ 240 Query: 241 DDINSDNAALAKFETIRAHGALRMGLIKHIDEAASRQHTPKIAFVAPPKSYASSSGKTVA 300 DDINSDNAALAKFETIRAHGA+RMGLIKHIDEAASRQHTPK+AFVA P SY SSSGK VA Sbjct: 241 DDINSDNAALAKFETIRAHGAVRMGLIKHIDEAASRQHTPKVAFVAKPTSYVSSSGKAVA 300 Query: 301 AEDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEKEAVRFGHPSGTL 360 AE++DLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEKEAVRFGHPSGTL Sbjct: 301 AEEIDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGEKEAVRFGHPSGTL 360 Query: 361 RVGAQAVQENGEWTVIKAIMSRSARVLMEGFVRVP 395 RVGAQA ENGEWTVIKAIMSRSARVLMEGFVRVP Sbjct: 361 RVGAQATLENGEWTVIKAIMSRSARVLMEGFVRVP 395 Lambda K H 0.318 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 650 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 397 Length of database: 397 Length adjustment: 31 Effective length of query: 366 Effective length of database: 366 Effective search space: 133956 Effective search space used: 133956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory