Align Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate 7026373 Shewana3_3515 ABC transporter related (RefSeq)
Query= TCDB::Q72L52 (376 letters) >FitnessBrowser__ANA3:7026373 Length = 349 Score = 178 bits (452), Expect = 2e-49 Identities = 111/292 (38%), Positives = 165/292 (56%), Gaps = 25/292 (8%) Query: 19 VKDFNLETEDGEFVVFVGPSGCGKTTTLRMIAGLEEISEGNIYIGDRLVND----VPPKD 74 +K +L GE + +GPSGCGKTT LR +AGL+ IS+G I I + V+ VP + Sbjct: 20 LKGLDLTLAQGEILALLGPSGCGKTTLLRAVAGLQAISQGEIQINGKTVSGAGQFVPSEQ 79 Query: 75 RDIAMVFQNYALYPHMNVYENMAFGLRLRRYPKDEIDRRVKEAARILKIEHLLNRKPREL 134 R I M+FQ+YAL+PH+ V EN+ FG+ + + R+ + ++K+E L R P EL Sbjct: 80 RGIGMIFQDYALFPHLTVAENILFGVA--KLTPAQRKARLDDMLALVKLEGLAKRYPHEL 137 Query: 135 SGGQRQRVAMGRAIVREPKVFLMDEPLSNLDAKLRVEMRAEIAKLQRRLGVTTIYVTHDQ 194 SGGQ+QRV++ RA+ EP++ L+DEP SN+DA++R M AEI + ++ V+ ++VTH + Sbjct: 138 SGGQQQRVSIARALAYEPQLLLLDEPFSNIDAQVRHSMMAEIRSILKQRNVSAVFVTHSK 197 Query: 195 VEAMTLGHRIVVMKDGEIQQVDTPLNLYDFPANRFVAGFIGSPSMNFVRAGVEVQGEKVY 254 EA + + G I Q NLY P +R+VA F+GS N++ A V + G V Sbjct: 198 DEAFVFADTLAIFNQGVIVQHGRAENLYAAPNSRYVADFLGSG--NYLPAEV-IDGHSV- 253 Query: 255 LVAPGFRIRANAVLGSALKPYAGKEVWLG---VRPEHLGLK----GYTTIPE 299 V P +R +L P + + G +RP+ L L G TI E Sbjct: 254 -VTPIGELR-------SLTPLSQSHAFNGQVFLRPQQLALSADDAGVGTITE 297 Score = 23.5 bits (49), Expect = 0.009 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%) Query: 325 NGTLLVAKV-DGHAPVKPGDKVELLADTQRLHAFD 358 +G L A+V DGH+ V P ++ L + HAF+ Sbjct: 239 SGNYLPAEVIDGHSVVTPIGELRSLTPLSQSHAFN 273 Lambda K H 0.320 0.139 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 307 Number of extensions: 10 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 376 Length of database: 349 Length adjustment: 29 Effective length of query: 347 Effective length of database: 320 Effective search space: 111040 Effective search space used: 111040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory