Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate 7023065 Shewana3_0303 short-chain dehydrogenase/reductase SDR (RefSeq)
Query= SwissProt::O18404 (255 letters) >FitnessBrowser__ANA3:7023065 Length = 253 Score = 117 bits (294), Expect = 2e-31 Identities = 74/197 (37%), Positives = 105/197 (53%), Gaps = 10/197 (5%) Query: 4 NAVSLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVAKELGDKVVFVPVDVTSEK 63 N ++VTG A G+G+A +L +GA V+ ADL + DK+V VDVT K Sbjct: 5 NKTAVVTGAAGGIGQAIIRKLLAEGARVVAADLSADALKVFNDFPADKLVTFSVDVTDYK 64 Query: 64 DVSAALQTAKDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVGTFNVI 123 V A ++ A D+FG+LD+ +N AG A + + DF+ V N+N G ++ I Sbjct: 65 QVEAMVKHAADQFGQLDILINNAGIGLAKPLLQHDP-----INDFEPVTNVNQKGVYHGI 119 Query: 124 RLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIARDLSTQ 183 L G + G RGVI+NT+SV A Y+ SKAAV MT A +L+ Sbjct: 120 -----LAGGRQFQAQGSRGVILNTSSVYAQIASEMTFTYNVSKAAVDMMTKCAALELAPL 174 Query: 184 GIRICTIAPGLFNTPML 200 G+R+C +APG +TPML Sbjct: 175 GVRVCAVAPGRVDTPML 191 Lambda K H 0.317 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 161 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 253 Length adjustment: 24 Effective length of query: 231 Effective length of database: 229 Effective search space: 52899 Effective search space used: 52899 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory