GapMind for catabolism of small carbon sources


Alignments for a candidate for maiA in Shewanella sp. ANA-3

Align glutathione transferase (EC; maleylacetoacetate isomerase (EC (characterized)
to candidate 7022758 Shewana3_0013 glutathione S-transferase domain-containing protein (RefSeq)

Query= BRENDA::O43708
         (216 letters)

          Length = 218

 Score = 48.5 bits (114), Expect = 9e-11
 Identities = 32/110 (29%), Positives = 56/110 (50%), Gaps = 5/110 (4%)

           LY   ++  SW +R  L  +  GI +  + + L  +    F +  ++++P  +VPTL   

            IT+  SL+I EY+ +   +    PQDPK++A  R  +  +  G   L+N

Lambda     K      H
   0.321    0.136    0.400 

Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 89
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 216
Length of database: 218
Length adjustment: 22
Effective length of query: 194
Effective length of database: 196
Effective search space:    38024
Effective search space used:    38024
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory