Align ABC transporter (characterized, see rationale)
to candidate 7026373 Shewana3_3515 ABC transporter related (RefSeq)
Query= uniprot:A0A166QFW2 (381 letters) >FitnessBrowser__ANA3:7026373 Length = 349 Score = 199 bits (505), Expect = 1e-55 Identities = 119/310 (38%), Positives = 182/310 (58%), Gaps = 16/310 (5%) Query: 4 LKLDNVNKQLGGMRILRDVSLEIAAGEFVVFVGPSGCGKSTLLRLIAGLDSICGGDLLID 63 L L V+ G ++L+ + L +A GE + +GPSGCGK+TLLR +AGL +I G++ I+ Sbjct: 5 LNLHQVHSDYQGQQVLKGLDLTLAQGEILALLGPSGCGKTTLLRAVAGLQAISQGEIQIN 64 Query: 64 GRRVND----LEPRERGVGMVFQSYALYPHMSVYDNISFGLKLAKTDKTSLRERVLKTAQ 119 G+ V+ + +RG+GM+FQ YAL+PH++V +NI FG+ AK + R+ Sbjct: 65 GKTVSGAGQFVPSEQRGIGMIFQDYALFPHLTVAENILFGV--AKLTPAQRKARLDDMLA 122 Query: 120 ILQLDKLLQRKPKELSGGQRQRVAMGRAMAREPDILLFDEPLSNLDASLRVQMRNEIARL 179 +++L+ L +R P ELSGGQ+QRV++ RA+A EP +LL DEP SN+DA +R M EI + Sbjct: 123 LVKLEGLAKRYPHELSGGQQQRVSIARALAYEPQLLLLDEPFSNIDAQVRHSMMAEIRSI 182 Query: 180 HDRLGSTMIYVTHDQVEAMTLADKIVVLNGGRVEQVGSPRELYERPASRFVAGFLGSPRM 239 + + ++VTH + EA AD + + N G + Q G LY P SR+VA FLGS Sbjct: 183 LKQRNVSAVFVTHSKDEAFVFADTLAIFNQGVIVQHGRAENLYAAPNSRYVADFLGSG-- 240 Query: 240 NFLSARLQTPGETSLVDTLVWGITSL-PFDSSNLAAGTPLSLGIRPEHVSLKAADGTAGV 298 N+L A + + V T + + SL P S+ G + +RP+ ++L A D G Sbjct: 241 NYLPAEVI---DGHSVVTPIGELRSLTPLSQSHAFNG---QVFLRPQQLALSADDAGVG- 293 Query: 299 VVTAVEYLGS 308 +T +LG+ Sbjct: 294 TITERRFLGA 303 Lambda K H 0.320 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 273 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 381 Length of database: 349 Length adjustment: 30 Effective length of query: 351 Effective length of database: 319 Effective search space: 111969 Effective search space used: 111969 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory