GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Slc28a3 in Shewanella sp. ANA-3

Align Broadly selective nucleoside:Na+ cotransporter, hfCNT (transports uridine, thymidine, inosine, 3'-azido-3'deoxythymidine, 2'3'dideoxycytidine, and 2'3'dideoxyinosine) (Na+/uridine = 2) (characterized)
to candidate 7023819 Shewana3_1039 Na+ dependent nucleoside transporter (RefSeq)

Query= TCDB::Q9UA35
         (683 letters)



>lcl|FitnessBrowser__ANA3:7023819 Shewana3_1039 Na+ dependent
           nucleoside transporter (RefSeq)
          Length = 419

 Score =  303 bits (775), Expect = 1e-86
 Identities = 158/416 (37%), Positives = 258/416 (62%), Gaps = 5/416 (1%)

Query: 194 NQVIPFFGLILYILLVFIFSKHPTKVRWRIVIWGLLLQFIFGLIILRTKPGLDAFNWLGI 253
           N ++   G+++ + + F+ S +   +  R V   L +Q  FG  +L    G D    +  
Sbjct: 2   NILMSLVGVVVLLAIGFLLSNNKKAINLRTVGGALAIQAAFGGFVLYVPVGKDILKSVSD 61

Query: 254 QVQTFLKYTDAGSRFLFGD--DFQDHF-FAFAVLPIVIFFSTVMSMMYYLGLMQWLILKV 310
            V + + Y   G  FLFGD  +F+  F FA  VLP+++FFS++++++YYLG+MQW+I  +
Sbjct: 62  AVSSVIGYAQNGIGFLFGDLANFKLGFIFAVNVLPVIVFFSSLIAVLYYLGIMQWIIRII 121

Query: 311 GWLMQITMGTSPMESMVSAGNIFVGQTESPLLIRPYLADLTISEMHSVMSSGFATIAGSV 370
           G  +Q  +GTS  ESM +  NIFVGQTE+PL++RP++  +T SE+ ++M  G A+IAGSV
Sbjct: 122 GGGLQKALGTSRTESMSATANIFVGQTEAPLVVRPFIPTMTQSELFAIMVGGLASIAGSV 181

Query: 371 LGAYISLGIPAAHLLTASVMSAPAALAISKTFWPETKKSKNSTQTSIKLEKGQENNLVEA 430
           L  Y  +G+P  +L+ AS M+AP  L ++K   PET+ +KN     +  +  +  N+++A
Sbjct: 182 LAGYAQMGVPIEYLVAASFMAAPGGLLMAKLMHPETEVAKND-MDELPEDPDKPANVLDA 240

Query: 431 ASQGASAAVPLVANIAANLIAFLAVLAFINATLSWLGSMFNYPQFSFEIICSYVLMPFAF 490
           A+ GAS+ + L  N+ A L+AF+ ++A IN  +  +G  F     + E+I  Y+ MP AF
Sbjct: 241 AAAGASSGMHLALNVGAMLLAFVGLIAMINGIIGGVGGWFGVEGLTLELILGYIFMPLAF 300

Query: 491 MMGVNYDDSFLVAELLGMKTFFNEFVAYQRLSEYIHNRESGGPLFVDGVRQYMSVRSEAI 550
           ++GV ++++ +    +G K   NEFVAY   + Y+ +   GG +  D     M+ R++AI
Sbjct: 301 LIGVPWNEALVAGSFIGQKIVVNEFVAYLNFAPYLKDIADGGMIVAD-TGLAMTDRTKAI 359

Query: 551 ATYALCGFANFGSLGIMIGGLSSLAPHRKSDIASCGIRALIAGTIACFSTACIAGV 606
            ++ALCGFAN  S+ I++GGL ++AP+R+ D+A  GIRA+IAG++A   +A IAG+
Sbjct: 360 ISFALCGFANLSSIAILLGGLGAMAPNRRHDLAKLGIRAVIAGSLANLMSATIAGL 415


Lambda     K      H
   0.325    0.139    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 584
Number of extensions: 20
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 683
Length of database: 419
Length adjustment: 35
Effective length of query: 648
Effective length of database: 384
Effective search space:   248832
Effective search space used:   248832
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory