Align trehalose-specific PTS system, I, HPr, and IIA components (characterized)
to candidate 7025893 Shewana3_3042 phosphoenolpyruvate-protein phosphotransferase PtsP (RefSeq)
Query= reanno::pseudo3_N2E3:AO353_15995 (844 letters) >FitnessBrowser__ANA3:7025893 Length = 744 Score = 291 bits (746), Expect = 7e-83 Identities = 199/564 (35%), Positives = 299/564 (53%), Gaps = 17/564 (3%) Query: 276 LLRGVCASAGSAFGYVVQVA-ERTLEMPEFAA-DQQLERESLERALMHATQALQRLR--- 330 L +G AS+G A + + + E +LE P+ D LE L A+ +A+ L Sbjct: 169 LFQGTSASSGIAIAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRF 228 Query: 331 DNAAGEAQADIFKAHQELLEDPSLLEQAQALIAEGKSAAFAWNSATEATATLFKSLGSTL 390 D E A IF A Q LL+D SL + + +G A A + + F ++ Sbjct: 229 DREQDEEVASIFNALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPY 288 Query: 391 LAERALDLMDVGQRVLKLILGVPDGVWELPDQ-AILIAEQLTPSQTAALDTGKVLGFATV 449 L ERA D+ D+GQ+VL+ ++ P+ + PD+ IL+ + + A K+ G T Sbjct: 289 LKERASDIRDLGQKVLRQLIE-PERLELEPDKPVILVTREADATMLAEFPRQKLAGIVTE 347 Query: 450 GGGATSHVAILARALGLPAVCGLPLQVLSLASGTRVLL-DADKGELHLDPAVSVIEQLHA 508 GG SH AILARALG+PA+ G+ Q+LS ++L+ +A +G+L + P+ +++ + + Sbjct: 348 LGGVNSHAAILARALGVPAITGVE-QLLSADIDQKLLVVNASRGQLMVSPSPAIVSEYRS 406 Query: 509 KRQQQRQRHQHELENAARAAVTRDGHHFEVTANVASLAETEQAMSLGAEGIGLLRSEFLY 568 Q+ + + A +V DG + N L+ ++ GA+GIGL R+E + Sbjct: 407 LISAQKALQRQYAQELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPF 466 Query: 569 QQRSVAPSHDEQAGTYSAIARALGPQRNLVVRTLDVGGDKPLAYVPMDSEANPFLGMRGI 628 + PS EQ Y + A R +V+RTLDVGGDKPL Y P+ E NPFLG RGI Sbjct: 467 MLQQRFPSESEQVKVYQQVLSAASG-RPVVMRTLDVGGDKPLPYFPI-KEDNPFLGWRGI 524 Query: 629 RLCLERPQLLREQFRAILSSAGLAR-LHIMLPMVSQLSELRLARLMLEEEALAL-----G 682 RL L+ P+L Q RA+L + G + L I+LPMVS L E+ + LE+ + L Sbjct: 525 RLSLDHPELFLVQLRAMLQAGGECKQLSILLPMVSNLDEIDQSLAYLEQAYVELKNDVNS 584 Query: 683 LRELPKLGIMIEVPAAALMADLFAPEVDFFSIGTNDLTQYTLAMDRDHPRLASQADSFHP 742 E+P++GIM+EVPA D A VDF S+G+NDLTQY LA+DR++PR++S DS+HP Sbjct: 585 QIEMPRIGIMLEVPALLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFDSYHP 644 Query: 743 SVLRLIASTVKAAHAHGKWVGVCGALASETLAVPLLLGLGVDELSVSVPLIPAIKAAIRE 802 +LR + H + +CG LA E + LL+ +G LS++ + I +R Sbjct: 645 GILRALHQARLDCDYHELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRR 704 Query: 803 VELSDCQAIAHQVLGLESAEQVRE 826 V +D + Q L L + QVRE Sbjct: 705 VSRADLTQLLSQALSLSNGFQVRE 728 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1160 Number of extensions: 53 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 844 Length of database: 744 Length adjustment: 41 Effective length of query: 803 Effective length of database: 703 Effective search space: 564509 Effective search space used: 564509 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory