Aligments for a candidate for acs in Shewanella sp. ANA-3

GapMind for catabolism of small carbon sources

Aligments for a candidate for acs in Shewanella sp. ANA-3

Align isobutanoate/2-methylbutanoate--CoA ligase (EC 6.2.1.1) (characterized)
to candidate 7025315 Shewana3_2475 AMP-binding domain protein (RefSeq)

Query= metacyc::MONOMER-20125
         (556 letters)



>FitnessBrowser__ANA3:7025315
          Length = 574

 Score =  163 bits (412), Expect = 2e-44
 Identities = 156/565 (27%), Positives = 254/565 (44%), Gaps = 81/565 (14%)

Query: 19  FLERAATVYGDCTSVVYDAVSYTWSQTHRRCLC----LASSIASLGIENGHVVSVLAPNV 74
           +L   A VY +  +VV +     W+  +R+ L     LA+ +  LGI  G  V + +PN 
Sbjct: 37  YLNDIANVYPEQLAVVVNHQDIRWN--YRQYLAQIDALATGLLKLGIGPGDRVGIWSPNN 94

Query: 75  PQMYELHFAVPMAGAILNAVNLRLDARTISILLHH-------------SESKLIFVDHLS 121
            +     FA    GAI+  +N       +   L +             S + L  +  L+
Sbjct: 95  IEWCLTQFATAKIGAIMVCINPAYRPEELQYALTNVGCRAVICADKFKSSNYLQMLYELA 154

Query: 122 RDLILEAIALFPKQAPVPRLVFMADESESGNSSELGKEFFCSYKDLIDRGDPDFKWVMPK 181
            +L + A      +A +P L F+       +   L      ++ DL+     D K  + +
Sbjct: 155 PELKVCAAGQLQAKA-LPDLQFVIRMGAEQSPGML------NFDDLLVEVTADDKAALER 207

Query: 182 -----SEWDPMILNYTSGTTSSPKGVVHCHRGIF---IMTVDSLIDWGVPKQPVYLWTLP 233
                S +D + + +TSGTT SPKG    H  I     +  +++      K  +    +P
Sbjct: 208 IAESLSPYDAINIQFTSGTTGSPKGATLSHHNILNNGYLVAEAMKFTCEDKLCI---PVP 264

Query: 234 MFHANGWSYPWGMAAVGGTNICLRK----------FDSEIIYDMIKRHGVTHMCGAPVVL 283
           ++H  G         V G  +CL K          FD     ++++R   T + G P + 
Sbjct: 265 LYHCFGM--------VLGNLVCLAKGAAAVFPGDSFDPLTTLEVVERERCTALHGVPTMF 316

Query: 284 NMLSNAPGSE-----PLKTTVQIMTAGAPPPSAVLFRTESLGFA--VSHGYGLTETAGLV 336
                 P  +      L+T V    AGA  P  V+ R ++L +   V  GYG TE + L 
Sbjct: 317 IAELEHPEFKRFDLSSLRTGVM---AGATCPEEVMRRVQNLMYMQEVLIGYGQTECSPL- 372

Query: 337 VSCAWKKEWNHLPATERARLKSRQGVGTVM-QTKIDVVDPVTGAAVKRDGSTLGEVVLRG 395
                    NH+   +    K    VG  +  T++ +VD   G  +  +  T GEV  RG
Sbjct: 373 ---------NHITEIDSPVEKRVLTVGRALPHTEVKIVDEF-GEVLPIN--TPGEVCSRG 420

Query: 396 GSVMLGYLKDPEGTAKSMTADGWFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEV 455
             +M  Y  DPE TA ++ ++GW ++GD+G M   GY++I  R KD+II GGEN+   E+
Sbjct: 421 YCIMQCYWNDPEKTAATIDSEGWLHSGDIGQMDEQGYVQIVGRIKDMIIRGGENIYPREI 480

Query: 456 ESILYSHPDILEAAVVARPDEFWGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVP 515
           E  LY+H D+ +AAV     + +GE  CA++ ++ G T   +E++I  +   K   + VP
Sbjct: 481 EEKLYTHKDVQDAAVFGVHSDKYGEEVCAWIKIRSGAT--ISEEDIRHFLTEKFAYFKVP 538

Query: 516 KTVVFKEELPKTSTGKVQKFILRDM 540
           + + F E+ P T TGK+QKF +R++
Sbjct: 539 RYIKFVEQYPMTVTGKIQKFKMREL 563


Lambda     K      H
   0.319    0.135    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 725
Number of extensions: 36
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 574
Length adjustment: 36
Effective length of query: 520
Effective length of database: 538
Effective search space:   279760
Effective search space used:   279760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources