Align Xylonate dehydratase (EC 4.2.1.82) (characterized)
to candidate 7023120 Shewana3_0358 dihydroxy-acid dehydratase (RefSeq)
Query= reanno::pseudo5_N2C3_1:AO356_28760 (594 letters) >FitnessBrowser__ANA3:7023120 Length = 619 Score = 210 bits (535), Expect = 1e-58 Identities = 176/579 (30%), Positives = 268/579 (46%), Gaps = 81/579 (13%) Query: 44 GRPIIGIAQTGSDLTPCNRHHLELAQRVKAGIRDAGGIPMEFPVHPIAEQSRRPTAAL-- 101 G+PII IA + + P + H ++ V I +AGGI EF + + + Sbjct: 33 GKPIIAIANSFTQFVPGHVHLKDMGSLVADAIEEAGGIAKEFNTIAVDDGIAMGHGGMLY 92 Query: 102 ---DRNLAYLGLVEILHGYPLDGVVLTTGCDKTTPACLMAAATTDLPAIVLSGGPMLDGH 158 R L + +++ + D +V + CDK TP LMAA ++P + +SGGPM G Sbjct: 93 SLPSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGK 152 Query: 159 HK--GELIGSGTVLWHARNLMAAGEIDYEGFMEMTTAASPSVGHCNTMGTALSMNALAEA 216 K +LI V A A + E ++ +A P+ G C+ M TA SMN L EA Sbjct: 153 TKLSDKLIKLDLV--DAMVAAADSSVSDEDSAKIERSACPTCGSCSGMFTANSMNCLTEA 210 Query: 217 LGMSLPGCASIPAPYRERGQMAYATGKRICELVLQ-------DIRPSQIMTRQAFENAIA 269 LG+SLPG S+ A + +R ++ G+R+ L + P I + +AFENA+A Sbjct: 211 LGLSLPGNGSMLATHADRRELFLEAGRRVMALTKRYYEQDDASALPRNIASFKAFENAMA 270 Query: 270 VASALGASSNCPPHLIAIARHMGVELSLDDWQRIGEDVPLLVNCMPA-GKYLGEGFHRAG 328 + A+G SSN HL+A A+ V ++DD R+ VP L P+ KY E HRAG Sbjct: 271 LDIAMGGSSNTVLHLLAAAQEADVAFTMDDIDRMSRQVPHLCKVAPSTAKYHMEDVHRAG 330 Query: 329 GVPSVMHELQKAGRLHEDCATVSGRTIGEIVSSSLTSNADVIHPFDTPLK---------- 378 GV ++ EL +AG LH D V+ G + S+ + DV+ D +K Sbjct: 331 GVMGILGELDRAGLLHTDVPHVAADAGGNL--KSVLAKYDVMQTQDDKVKQFFMAGPAGI 388 Query: 379 --------------------------------HRAGFIVLSGNFFDS-AIMKMSVVGEAF 405 G VLSGN D+ I+K + V E Sbjct: 389 PTTKAFSQDCRWPSLDDDRREGCIRSREFAFSQEGGLAVLSGNLADNGCIVKTAGVDE-- 446 Query: 406 RKTYLSEPGAENSFEARAIVFEGPEDYHARIDDPALDIDERCILVIRGVGTVGYPGSAEV 465 + +F A V+E +D A I + + ++VIR G G PG E+ Sbjct: 447 ---------SNLTFIGSARVYESQDDAVAGILGGEVVAGD--VVVIRYEGPKGGPGMQEM 495 Query: 466 VNMAPPAALIKQGI-DSLPCLGDGRQSGTSASPSILNMSPEAAVGGGLALLQTNDRLKVD 524 + P + L +G+ + + DGR SG ++ SI ++SPEAA GG +AL++ DR+++D Sbjct: 496 --LYPTSYLKSRGLGKACALITDGRFSGGTSGLSIGHVSPEAAAGGTIALIENGDRIEID 553 Query: 525 LNTRTVNLLIDDEEMARRRLEWTPNIPPSQTPWQELYRQ 563 + R++ L + D E+A RR P + W+ L RQ Sbjct: 554 IPKRSIKLAVSDAELAARRETMLARGPMA---WKPLSRQ 589 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 827 Number of extensions: 42 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 594 Length of database: 619 Length adjustment: 37 Effective length of query: 557 Effective length of database: 582 Effective search space: 324174 Effective search space used: 324174 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory