4-hydroxybenzoate catabolism in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

4-hydroxybenzoate catabolism in Burkholderia phytofirmans PsJN

Best path

pcaK, pobA, pcaH, pcaG, pcaB, pcaC, pcaD, pcaI, pcaJ, pcaF

Also see fitness data for the top candidates

Rules

Overview: 4-hydroxybenzoate catabolism in GapMind is based on aerobic oxidation to 3,4-hydroxybenzoate (protocatechuate), followed by meta, ortho, or para cleavage; or reduction to benzoyl-CoA (part of a MetaCyc pathway for phenol degradation, link)

all:

4-hydroxybenzoate-transport, pobA and protocatechuate-degradation
or 4-hydroxybenzoate-transport, hcl, hcrA, hcrB, hcrC and benzoyl-CoA-degradation
Comment: An aerobic route for degradation of 4-hydroxybenzoate involves 4-hydroxybenzoate 3-monooxygenase pobA, which forms protocatechuate (3,4-dihydroxybenzoate). Alternatively, 4-hydroxybenzoate can be activated to 4-hydroxybenzoyl-CoA by hcl and reduced to benzoyl-CoA by hcrABC (link).

protocatechuate-degradation:

ligA, ligB, ligC, ligI, ligU, ligJ and ligK
or pcaH, pcaG, pcaB, pcaC, pcaD and 3-oxoadipate-degradation
or praA and 2-hydroxymuconate-6-semialdehyde-degradation
Comment: In the meta-cleavage pathway (link), the 4,5-dioxygenase ligAB splits protocatechuate to 4-carboxy-2-hydroxymuconate-6-semialdehyde. (In solution, this is in the hemiacetal form.) The semialdehyde is oxidized to 2-pyrone-4,6-dicarboxylate, hydrolyzed to (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate, tautomerized to (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate, hydrated to 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate (4-oxalocitramalate), and split by an aldolase to pyruvate and oxaloacetate. In the ortho-cleavage pathway (link), the 3,4-oxygenase pcaHG cleaves the ring to 3-carboxy-cis,cis-muconate, a cycloisomerase forms 4-carboxymuconolactone (2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate), a decarboxylase forms 3-oxoadipate enol lactone ((4,5-dihydro-5-oxofuran-2-yl)-acetate), and a hydrolase forms 3-oxoadipate. In the para-cleavage pathway (link), the 2,3-dioxygenase praA forms (2Z,4Z)-2-hydroxy-5-carboxymuconate-6-semialdehyde, which spontaneously decarboxylates to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate, also known as 2-hydroxymuconate 6-semialdehyde.

2-hydroxymuconate-6-semialdehyde-degradation:

praB, praC, praD and 2-hydroxypenta-2,4-dienoate-degradation
or xylF and 2-hydroxypenta-2,4-dienoate-degradation
Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (This series of steps is part of protocatechuate para-cleavage, link, or catechol degradation II, link.) Or, hydrolase xylF forms HPD and formate. (This is part of a MetaCyc pathway for catechol degradation, link.)

benzoyl-CoA-degradation:

benzoyl-CoA-reductase, dch, had, oah, pimB and glutaryl-CoA-degradation
or benzoyl-CoA-reductase, Ch1CoA, badK, badH, badI, pimD, pimC, pimF and glutaryl-CoA-degradation
or boxA, boxB, boxC, boxD, paaF, paaH and paaJ2
Comment: Benzoyl-CoA can be degraded anaerobically (link) by reduction to cyclohex-1,5-diene-1-carbonyl-CoA, followed by hydratase (dch) to 6-hydroxycyclohex-1-ene-1-carbonyl-CoA, a dehydrogenase to 6-oxocyclohex-1-ene-1-carbonyl-CoA, a hydrolase to 2-hydroxy-6-oxocycloheane-1-carbonyl-CoA, a ring-opening hydrolase to 3-hydroxypimeloyl-CoA [the last two steps are both catalyzed by oah], a dehydrogenase to 3-oxopimeloyl-CoA [not linked to sequence and omitted], and an acetyltransferase to glutaryl-CoA and acetyl-CoA. Alternatively, after reduction to cyclohex-1,5-diene-1-carbonyl-CoA, Ch1CoA can further reduce it to cyclohex-1-ene-1-carboxyl-CoA (link), followed by hydration to 2-hydroxy-cyclohexane-1-carbonyl-CoA, oxidation to 2-ketocyclohexane-1-carbonyl-CoA, cleavage by a ring-opening hydrolase to pimeloyl-CoA, oxidation to 2,3-didehydropimeloyl-CoA, hydration to 3-hydroxypimeloyl-C, oxidation to 3-oxopimeloyl-CoA and cleavage by a thiolase to glutaryl-CoA and acetyl-CoA. Benzoyl-CoA degradation can be degraded aerobically (link) by an epoxidase (boxAB) that forms 2,3-epoxy-2-3-dihydrobenzoyl-CoA; a dihydrolase forms cis-3,4-dihydroadipyl-CoA semialdehyde and formate; a dehydrogenase forms cis-3,4-dehydroadipyl-CoA; and an unknown isomerase forms trans-2,3-dehydroadipyl-CoA. This is converted to succinyl-CoA as in the anaerobic pathway (paaF, paaH, and paaJ2).

glutaryl-CoA-degradation: gcdH, ech, fadB and atoB

Comment: In MetaCyc pathway glutaryl-CoA degradation (link), glutaryl-CoA is oxidized to (E)-glutaconyl-CoA and oxidatively decarboxylated to crotonyl-CoA (both by the same enzyme), hydrated to 3-hydroxybutanoyl-CoA, oxidized to acetoacetyl-CoA, and cleaved to two acetyl-CoA.

benzoyl-CoA-reductase:

bcrA, bcrB, bcrC and bcrD
or bamB, bamC, bamD, bamE, bamF, bamG, bamH and bamI
Comment: Benzoyl-CoA reduction is energetically unfavorable. There are two classes of reductases: class I enzymes (bcrABCD) use ATP to drive the reaction, while class II enzymes (bamBCDEFGHI) are thought to us an electron bifurcation. SYN_02587 (Q2LQN9) from Syntrophus aciditrophicus, which can oxidize cyclohex-1,5-diene-1-carbonyl-CoA to benzoyl-CoA, is not included because it seems to lack a mechanism to drive benzoyl-CoA reduction.

2-hydroxypenta-2,4-dienoate-degradation: mhpD, mhpE and acetaldehyde-degradation

Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. In MetaCyc pathway 2-hydroxypenta-2,4-dienoate degradation (link), HPD is hydrated to (S)-4-hydroxy-2-oxopentanoate and an aldolase cleaves it to pyruvate and acetaldehyde.

acetaldehyde-degradation:

ald-dh-CoA
or adh and acs
or adh, ackA and pta
Comment: Acetaldehyde can be oxidized to acetyl-CoA, or oxidized to acetate and activated to acetyl-CoA by either acetyl-CoA synthetase (acs) or by acetate kinase (ackA) and phosphate acetyltransferase (pta).

3-oxoadipate-degradation: 3-oxodipate-CoA-transferase and pcaF

Comment: MetaCyc pathway 3-oxoadipate degradation (link) involves activation by CoA (using succinyl-CoA) and a thiolase (succinyltransferase) reaction that splits it to acetyl-CoA and succinyl-CoA.

3-oxodipate-CoA-transferase:

pcaI and pcaJ
or catI and catJ
Comment: Two different types of 3-oxoadipate CoA-transferases (EC 2.8.3.6) are known. They are both heteromeric with each subunit containing a CoA-transferase domain

4-hydroxybenzoate-transport:

pcaK
or fcbT1, fcbT2 and fcbT3
Comment: Transporters were identified using: query: transporter:4-hydroxybenzoate:p-hydroxybenzoate:4-hydroxybenzoic acid:p-hydroxybenzoic acid

72 steps (53 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
pcaK	4-hydroxybenzoate transporter pcaK	BPHYT_RS30250	BPHYT_RS03795
pobA	4-hydroxybenzoate 3-monooxygenase	BPHYT_RS11060
pcaH	protocatechuate 3,4-dioxygenase, alpha subunit	BPHYT_RS33460	BPHYT_RS33455
pcaG	protocatechuate 3,4-dioxygenase, beta subunit	BPHYT_RS33455
pcaB	3-carboxymuconate cycloisomerase	BPHYT_RS21425	BPHYT_RS14615
pcaC	4-carboxymuconolactone decarboxylase	BPHYT_RS21435	BPHYT_RS21430
pcaD	3-oxoadipate enol-lactone hydrolase	BPHYT_RS21430	BPHYT_RS02320
pcaI	3-oxoadipate CoA-transferase subunit A (PcaI)	BPHYT_RS21415	BPHYT_RS13675
pcaJ	3-oxoadipate CoA-transferase subunit B (PcaJ)	BPHYT_RS21420	BPHYT_RS13670
pcaF	succinyl-CoA:acetyl-CoA C-succinyltransferase	BPHYT_RS29385	BPHYT_RS17345
Alternative steps:
ackA	acetate kinase	BPHYT_RS06125	BPHYT_RS26200
acs	acetyl-CoA synthetase, AMP-forming	BPHYT_RS07000	BPHYT_RS27780
adh	acetaldehyde dehydrogenase (not acylating)	BPHYT_RS25810	BPHYT_RS00120
ald-dh-CoA	acetaldehyde dehydrogenase, acylating	BPHYT_RS07245	BPHYT_RS21770
atoB	acetyl-CoA C-acetyltransferase	BPHYT_RS09150	BPHYT_RS09180
badH	2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase	BPHYT_RS30440	BPHYT_RS04770
badI	2-ketocyclohexanecarboxyl-CoA hydrolase	BPHYT_RS28620	BPHYT_RS17335
badK	cyclohex-1-ene-1-carboxyl-CoA hydratase	BPHYT_RS17335	BPHYT_RS17350
bamB	class II benzoyl-CoA reductase, BamB subunit
bamC	class II benzoyl-CoA reductase, BamC subunit
bamD	class II benzoyl-CoA reductase, BamD subunit	BPHYT_RS25200
bamE	class II benzoyl-CoA reductase, BamE subunit
bamF	class II benzoyl-CoA reductase, BamF subunit
bamG	class II benzoyl-CoA reductase, BamG subunit
bamH	class II benzoyl-CoA reductase, BamH subunit	BPHYT_RS06650	BPHYT_RS14990
bamI	class II benzoyl-CoA reductase, BamI subunit	BPHYT_RS14995	BPHYT_RS06655
bcrA	ATP-dependent benzoyl-CoA reductase, alpha subunit
bcrB	ATP-dependent benzoyl-CoA reductase, beta subunit
bcrC	ATP-dependent benzoyl-CoA reductase, gamma subunit
bcrD	ATP-dependent benzoyl-CoA reductase, delta subunit
boxA	benzoyl-CoA epoxidase, subunit A	BPHYT_RS13335
boxB	benzoyl-CoA epoxidase, subunit B	BPHYT_RS13340
boxC	2,3-epoxybenzoyl-CoA dihydrolase	BPHYT_RS13345
boxD	3,4-dehydroadipyl-CoA semialdehyde dehydrogenase	BPHYT_RS13360
catI	3-oxoadipate CoA-transferase subunit A (CatI)
catJ	3-oxoadipate CoA-transferase subunit B (CatJ)
Ch1CoA	cyclohex-1-ene-1-carbonyl-CoA dehydrogenase	BPHYT_RS20780	BPHYT_RS28040
dch	cyclohexa-1,5-diene-1-carboxyl-CoA hydratase	BPHYT_RS17335	BPHYT_RS10815
ech	(S)-3-hydroxybutanoyl-CoA hydro-lyase	BPHYT_RS17335	BPHYT_RS28020
fadB	(S)-3-hydroxybutanoyl-CoA dehydrogenase	BPHYT_RS13545	BPHYT_RS03225
fcbT1	tripartite 4-hydroxybenzoate transporter, substrate-binding component FcbT1
fcbT2	tripartite 4-hydroxybenzoate transporter, small DctQ-like component FcbT2
fcbT3	tripartite 4-hydroxybenzoate transporter, large permease subunit FcbT3	BPHYT_RS30450
gcdH	glutaryl-CoA dehydrogenase	BPHYT_RS03780	BPHYT_RS23260
had	6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
hcl	4-hydroxybenzoyl-CoA ligase	BPHYT_RS13365	BPHYT_RS35445
hcrA	4-hydroxybenzoyl-CoA reductase, alpha subunit	BPHYT_RS01765	BPHYT_RS10835
hcrB	4-hydroxybenzoyl-CoA reductase, beta subunit	BPHYT_RS29710	BPHYT_RS20915
hcrC	4-hydroxybenzoyl-CoA reductase, gamma subunit	BPHYT_RS01770	BPHYT_RS10825
ligA	protocatechuate 4,5-dioxygenase, alpha subunit
ligB	protocatechuate 4,5-dioxygenase, beta subunit
ligC	2-hydroxy-4-carboxymuconate-6-semialdehyde dehydrogenase
ligI	2-pyrone-4,6-dicarboxylate hydrolase	BPHYT_RS35395	BPHYT_RS00910
ligJ	4-carboxy-2-hydroxymuconate hydratase	BPHYT_RS30325
ligK	4-oxalocitramalate aldolase	BPHYT_RS12315	BPHYT_RS30330
ligU	4-oxalomesaconate tautomerase	BPHYT_RS30140
mhpD	2-hydroxypentadienoate hydratase	BPHYT_RS07240	BPHYT_RS28900
mhpE	4-hydroxy-2-oxovalerate aldolase	BPHYT_RS07250	BPHYT_RS28905
oah	6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase
paaF	2,3-dehydroadipyl-CoA hydratase	BPHYT_RS17335	BPHYT_RS25360
paaH	3-hydroxyadipyl-CoA dehydrogenase	BPHYT_RS13545	BPHYT_RS03225
paaJ2	3-oxoadipyl-CoA thiolase	BPHYT_RS17345	BPHYT_RS29385
pimB	3-oxopimeloyl-CoA:CoA acetyltransferase	BPHYT_RS09565	BPHYT_RS03230
pimC	pimeloyl-CoA dehydrogenase, small subunit	BPHYT_RS13555	BPHYT_RS25385
pimD	pimeloyl-CoA dehydrogenase, large subunit	BPHYT_RS06715	BPHYT_RS13550
pimF	6-carboxyhex-2-enoyl-CoA hydratase	BPHYT_RS09560	BPHYT_RS13545
praA	protocatechuate 2,3-dioxygenase
praB	2-hydroxymuconate 6-semialdehyde dehydrogenase	BPHYT_RS07235	BPHYT_RS28885
praC	2-hydroxymuconate tautomerase	BPHYT_RS24200	BPHYT_RS10700
praD	2-oxohex-3-enedioate decarboxylase	BPHYT_RS07255	BPHYT_RS28900
pta	phosphate acetyltransferase	BPHYT_RS21700	BPHYT_RS27695
xylF	2-hydroxymuconate semialdehyde hydrolase	BPHYT_RS23610

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources