Align 3-oxoadipyl-CoA thiolase; EC 2.3.1.174 (characterized, see rationale)
to candidate BPHYT_RS29385 BPHYT_RS29385 acetyl-CoA acetyltransferase
Query= uniprot:B2SYZ2 (400 letters) >FitnessBrowser__BFirm:BPHYT_RS29385 Length = 400 Score = 650 bits (1678), Expect = 0.0 Identities = 319/400 (79%), Positives = 360/400 (90%) Query: 1 MNDAFICDAIRTPIGRYGGALKDVRADDLGAVPIKALIERNPGVDWRTVDDVIYGCANQA 60 M +AF+CDAIRTPIGRY G+L VRADDLGAVP+KAL+ERN VDW +DDVIYGCANQA Sbjct: 1 MTEAFLCDAIRTPIGRYAGSLSSVRADDLGAVPLKALMERNKDVDWNAIDDVIYGCANQA 60 Query: 61 GEDNRNVARMSALLAGLPVEAPGSTINRLCGSGMDAVGTAARAIKAGEARLMIAGGVESM 120 GEDNRNVARMS LLAGLP PG+T+NRLCGSGMDAVG AARAIK+GEA LM+AGGVESM Sbjct: 61 GEDNRNVARMSLLLAGLPQGVPGTTVNRLCGSGMDAVGIAARAIKSGEAALMVAGGVESM 120 Query: 121 TRAPFVMGKATSAFARQADIYDTTIGWRFINPLMKRQYGVDSMPETAENVAAEFSVSRAD 180 +RAPFVMGKA +AF+RQA+IYDTTIGWRF+NPLMK+ YGVDSMPET ENVA ++++SRAD Sbjct: 121 SRAPFVMGKAPTAFSRQAEIYDTTIGWRFVNPLMKKLYGVDSMPETGENVATDYNISRAD 180 Query: 181 QDAFALASQQKAARAQQDGTLAQEIVGVEIAQKKGDPVRALLDEHPRETSLESLGKLKGV 240 QDAFA+ SQQKAARAQ+DGTLAQEIVGV IAQKKGDP+ L DEHPRETSLE+L KLKGV Sbjct: 181 QDAFAVRSQQKAARAQRDGTLAQEIVGVTIAQKKGDPITVLQDEHPRETSLETLAKLKGV 240 Query: 241 VRPDGSVTAGNASGVNDGACALLLANQQAADQYGLRRRARVVGMATAGVEPRIMGIGPAP 300 VRPDG+VTAGNASGVNDGA ALLLAN++ A ++GL RARV+G+ATAGV PR+MGIGPAP Sbjct: 241 VRPDGTVTAGNASGVNDGAAALLLANEETARRFGLTPRARVLGIATAGVAPRVMGIGPAP 300 Query: 301 ATQKLLKQLGMTLEQLDVIELNEAFASQGLAVLRTLGLRDDDPRVNPNGGAIALGHPLGA 360 ATQKLL +L MT++Q DVIELNEAFASQG+AVLR LG+ DDD RVNPNGGAIALGHPLG Sbjct: 301 ATQKLLARLNMTIDQFDVIELNEAFASQGIAVLRALGVADDDARVNPNGGAIALGHPLGM 360 Query: 361 SGARLITTALYQLERTNGRFALCTMCIGVGQGIALVIERL 400 SGARL+TTA+YQL RT GRFALCTMCIGVGQGIA+ IER+ Sbjct: 361 SGARLVTTAMYQLHRTQGRFALCTMCIGVGQGIAIAIERV 400 Lambda K H 0.318 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 599 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 400 Length adjustment: 31 Effective length of query: 369 Effective length of database: 369 Effective search space: 136161 Effective search space used: 136161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate BPHYT_RS29385 BPHYT_RS29385 (acetyl-CoA acetyltransferase)
to HMM TIGR02430 (pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02430.hmm # target sequence database: /tmp/gapView.16435.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02430 [M=400] Accession: TIGR02430 Description: pcaF: 3-oxoadipyl-CoA thiolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-225 734.1 11.1 2.3e-225 734.0 11.1 1.0 1 lcl|FitnessBrowser__BFirm:BPHYT_RS29385 BPHYT_RS29385 acetyl-CoA acetylt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__BFirm:BPHYT_RS29385 BPHYT_RS29385 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 734.0 11.1 2.3e-225 2.3e-225 2 400 .] 3 400 .] 2 400 .] 1.00 Alignments for each domain: == domain 1 score: 734.0 bits; conditional E-value: 2.3e-225 TIGR02430 2 evyivdairtpiGrygGslssvraddlaavplkallarnpsldaaaiddvilGcanqaGednrnvarmaa 71 e++++dairtpiGry+Gslssvraddl+avplkal++rn+++d++aiddvi+GcanqaGednrnvarm++ lcl|FitnessBrowser__BFirm:BPHYT_RS29385 3 EAFLCDAIRTPIGRYAGSLSSVRADDLGAVPLKALMERNKDVDWNAIDDVIYGCANQAGEDNRNVARMSL 72 89******************************************************************** PP TIGR02430 72 llaGlpvsvpgttvnrlcgsgldalglaaraikaGeadlviaGGvesmsrapfvlGkadsafsrsakled 141 llaGlp+ vpgttvnrlcgsg+da+g+aaraik+Gea l++aGGvesmsrapfv+Gka +afsr+a++ d lcl|FitnessBrowser__BFirm:BPHYT_RS29385 73 LLAGLPQGVPGTTVNRLCGSGMDAVGIAARAIKSGEAALMVAGGVESMSRAPFVMGKAPTAFSRQAEIYD 142 ********************************************************************** PP TIGR02430 142 ttiGwrfvnpklkalyGvdsmpetaenvaeefgvsredqdafalrsqqrtaaaqakGffaeeivpveikq 211 ttiGwrfvnp +k+lyGvdsmpet enva+++++sr+dqdafa+rsqq++a+aq G +a+eiv v+i+q lcl|FitnessBrowser__BFirm:BPHYT_RS29385 143 TTIGWRFVNPLMKKLYGVDSMPETGENVATDYNISRADQDAFAVRSQQKAARAQRDGTLAQEIVGVTIAQ 212 ********************************************************************** PP TIGR02430 212 kkGeetvvdkdehlraettlealaklkavvredgtvtaGnasGvndGaaalllaseeavkrhgltprari 281 kkG++ +v +deh+r et+le+laklk+vvr+dgtvtaGnasGvndGaaallla ee+++r+gltprar+ lcl|FitnessBrowser__BFirm:BPHYT_RS29385 213 KKGDPITVLQDEHPR-ETSLETLAKLKGVVRPDGTVTAGNASGVNDGAAALLLANEETARRFGLTPRARV 281 ***************.****************************************************** PP TIGR02430 282 laaasaGveprvmGlgpvpavkkllaraglsledldvielneafaaqalavlrelgladddarvnpnGGa 351 l++a+aGv+prvmG+gp+pa++kllar++++++++dvielneafa+q++avlr+lg+adddarvnpnGGa lcl|FitnessBrowser__BFirm:BPHYT_RS29385 282 LGIATAGVAPRVMGIGPAPATQKLLARLNMTIDQFDVIELNEAFASQGIAVLRALGVADDDARVNPNGGA 351 ********************************************************************** PP TIGR02430 352 ialGhplGasGarlvltalkqleksggryalatlciGvGqGialvierv 400 ialGhplG+sGarlv+ta++ql++++gr+al+t+ciGvGqGia++ierv lcl|FitnessBrowser__BFirm:BPHYT_RS29385 352 IALGHPLGMSGARLVTTAMYQLHRTQGRFALCTMCIGVGQGIAIAIERV 400 ************************************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (400 nodes) Target sequences: 1 (400 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 6.71 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory