Alignments for a candidate for araG in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for araG in Burkholderia phytofirmans PsJN

Align L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 (characterized)
to candidate BPHYT_RS27185 BPHYT_RS27185 D-ribose transporter ATP-binding protein

Query= CharProtDB::CH_014279
         (504 letters)



>FitnessBrowser__BFirm:BPHYT_RS27185
          Length = 516

 Score =  384 bits (986), Expect = e-111
 Identities = 212/490 (43%), Positives = 300/490 (61%), Gaps = 3/490 (0%)

Query: 4   STPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGS 63
           S   L  +G+ K FPGV AL  I  D  AG+VHA+ GENGAGKSTL+KI+SG Y    G 
Sbjct: 20  SREILQLKGVSKRFPGVVALDGIDLDLCAGEVHAVCGENGAGKSTLMKIISGQYRADEGV 79

Query: 64  VVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYE 123
           V   G  + FS T+ A  AG+AII+QEL+LVP ++VAENIYL + P +G  V+   LN  
Sbjct: 80  VRYRGAPVQFSSTSDAQAAGIAIIHQELNLVPHLSVAENIYLAREPKRGPFVDYRTLNSN 139

Query: 124 AGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLF 183
           A   L+ +G+++ P T +  LS+ Q QMVEIAKAL+ +A+++  DEPTSSL+  E   LF
Sbjct: 140 AQRCLQRIGLNVSPSTLVGALSLAQQQMVEIAKALSLDARVLIMDEPTSSLTESETVQLF 199

Query: 184 RVIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGR 243
           R+IRELR  G  ILY+SHR++E+  + D +TV +DGR++ T +D      + +V  MVGR
Sbjct: 200 RIIRELRAGGVAILYISHRLDEMAEIVDRVTVLRDGRHIAT-SDFASTTVNEIVARMVGR 258

Query: 244 DIGDIY-GWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLVGAGRSELMKGM 302
            + D Y   Q     +  LR+  ++  GV  P+S  +R GEI+G  GL+GAGR+E  + +
Sbjct: 259 PLDDAYPPRQSTPSNQILLRVRDLQRTGVFGPLSFELRKGEILGFAGLMGAGRTETARAI 318

Query: 303 FGGTQITAGQVYIDQQPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSVRDNINISARRK 362
           FG  +  +G + +  +P+ I  P  AI  G+    EDRK +G+     V  NI + A  +
Sbjct: 319 FGAERPDSGSITLGDEPVTIGSPREAIRHGIAYLSEDRKKDGLALSMPVSANITL-ANVR 377

Query: 363 HVLGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKVIL 422
            +     +    E   A+ ++R L I+TP  +Q+  NLSGGNQQK ++ +WL    +++ 
Sbjct: 378 AISSRGFLRFSEETAIAERYVRELGIRTPTVKQIARNLSGGNQQKIVISKWLYRGSRILF 437

Query: 423 LDEPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEIAGELL 482
            DEPTRGIDVGAK+ IY ++  LAA GV V+  SS+LPE+LG+ DRI V  EG I   L 
Sbjct: 438 FDEPTRGIDVGAKYAIYGLMDRLAADGVGVVLISSELPELLGMTDRIAVFHEGRITAVLE 497

Query: 483 HEQADERQAL 492
             Q  + + L
Sbjct: 498 TRQTSQEEIL 507



 Score = 71.6 bits (174), Expect = 6e-17
 Identities = 53/227 (23%), Positives = 106/227 (46%), Gaps = 13/227 (5%)

Query: 26  ISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVA 85
           +SF+   G++    G  GAG++   + + G   P +GS+ +  + ++      A+  G+A
Sbjct: 291 LSFELRKGEILGFAGLMGAGRTETARAIFGAERPDSGSITLGDEPVTIGSPREAIRHGIA 350

Query: 86  IIYQELH---LVPEMTVAENIYLGQLPHKGGIVNRSLLNYE-----AGLQLKHLGMDIDP 137
            + ++     L   M V+ NI L  +     I +R  L +      A   ++ LG+    
Sbjct: 351 YLSEDRKKDGLALSMPVSANITLANVR---AISSRGFLRFSEETAIAERYVRELGIRTPT 407

Query: 138 DTPL-KYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVI 196
              + + LS G  Q + I+K L R ++I+ FDEPT  +       ++ ++  L  +G  +
Sbjct: 408 VKQIARNLSGGNQQKIVISKWLYRGSRILFFDEPTRGIDVGAKYAIYGLMDRLAADGVGV 467

Query: 197 LYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGR 243
           + +S  + E+  ++D I VF +GR +    + +Q   + ++    GR
Sbjct: 468 VLISSELPELLGMTDRIAVFHEGR-ITAVLETRQTSQEEILHHASGR 513


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 650
Number of extensions: 19
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 504
Length of database: 516
Length adjustment: 34
Effective length of query: 470
Effective length of database: 482
Effective search space:   226540
Effective search space used:   226540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources