GapMind for catabolism of small carbon sources

 

Aligments for a candidate for lhgD in Burkholderia phytofirmans PsJN

Align L-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.2 (characterized)
to candidate BPHYT_RS01855 BPHYT_RS01855 FAD-dependent oxidoreductase

Query= SwissProt::Q9LES4
         (483 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS01855 BPHYT_RS01855 FAD-dependent
           oxidoreductase
          Length = 368

 Score =  368 bits (945), Expect = e-106
 Identities = 193/400 (48%), Positives = 260/400 (65%), Gaps = 40/400 (10%)

Query: 78  ERVDTVVIGAGVVGLAVARELSLRGREVLILDAASSFGTVTSSRNSEVVHAGIYYPPNSL 137
           ++++ VVIGAGV+GLAVAR L+ RGR+V++L+AA + G  TSSRNSEV+HAGIYYP  SL
Sbjct: 2   DQIECVVIGAGVIGLAVARALAARGRDVIVLEAAEAIGVGTSSRNSEVIHAGIYYPRGSL 61

Query: 138 KAKFCVRGRELLYKYCSEYEIPHKKIGKLIVATGSSEIPKLDLLMHLGTQNRVSGLRMLE 197
           KA  CVRGRE+LY YC E  +PH + GKL+VAT  ++IP+L+ +M  G  N V  L  + 
Sbjct: 62  KAALCVRGREMLYDYCVERNVPHSRCGKLLVATSRNQIPQLESIMAKGRDNGVLDLMRIS 121

Query: 198 GFEAMRMEPQLRCVKALLSPESGILDTHSFMLSLVEKSFDFMVYRDNNNLRLQGEAQNNH 257
           G +A  +EP L CV+A+ SP++GI+D+H  ML+                  LQG+A+ + 
Sbjct: 122 GDQAQALEPALECVEAVFSPQTGIVDSHQLMLA------------------LQGDAERDG 163

Query: 258 ATFSYNTVVLNGRVEEKKMHLYVADTRFSESRCEAEAQLELIPNLVVNSAGLGAQALAKR 317
           A  +++  V        +  + V             A   +    V+NSAGL A ALA+R
Sbjct: 164 AVCAFHAPVKAIEASNGRFIINVG----------GGAPTTISAACVINSAGLQANALARR 213

Query: 318 LHGLDHRFVPSSHYARGCYFTLSGIKAPPFNKLVYPIPEEGGLGVHVTVDLNGLVKFGPD 377
           + GLD R VP  + ARG YF++SG    PF++L+YP+P E GLGVH+T+DL G  +FGPD
Sbjct: 214 IRGLDARHVPPLYLARGNYFSISG--RAPFSRLIYPMPNEAGLGVHLTIDLGGQARFGPD 271

Query: 378 VEWIECTDDTSSFLNKFDYRVNPQRSEKFYPEIRKYYPDLKDGSLEPGYSGIRPKLSGPK 437
           VEW++            +Y V+P+R+E FY  IR Y+P L D +L+P Y+GIRPKLSGP 
Sbjct: 272 VEWVDA----------INYDVDPRRAESFYAAIRTYWPALPDDALQPAYAGIRPKLSGPG 321

Query: 438 QSPADFVIQGEETHGVPGLVNLFGIESPGLTSSLAIAEHI 477
           +  ADFVIQG   HGV GLVNLFGIESPGLT+SLAIA+ +
Sbjct: 322 EPAADFVIQGPAAHGVRGLVNLFGIESPGLTASLAIAQRV 361


Lambda     K      H
   0.318    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 494
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 368
Length adjustment: 32
Effective length of query: 451
Effective length of database: 336
Effective search space:   151536
Effective search space used:   151536
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory