Align Primary amine oxidase 1; AtAO1; EC 1.4.3.21 (characterized)
to candidate BPHYT_RS09910 BPHYT_RS09910 tyramine oxidase
Query= SwissProt::O23349 (650 letters) >FitnessBrowser__BFirm:BPHYT_RS09910 Length = 661 Score = 261 bits (668), Expect = 5e-74 Identities = 195/653 (29%), Positives = 309/653 (47%), Gaps = 57/653 (8%) Query: 22 FHPLDPLTPQEINKTSFIVKKSHLGNLKDLTFHYLDLEEPNKSHVLQWLSPNPSKKPPPP 81 FHPLDPL+ E+ +VK + + F ++L EP K+ V+ + K Sbjct: 13 FHPLDPLSGAEMQLACDLVKAAEKLD-SHARFPMVELREPPKAEVVAF------KTGEYF 65 Query: 82 RRRSFVVV--RAGGQTYELIIDLTTSKIASSRIY----TGHGFPSFTFIELFKASKLPLT 135 R +FV+ R G T E +DL KIA+ R+ +G P + A ++ + Sbjct: 66 SRTAFVLAIDRTNGATIEFEVDLREKKIAARRVMPFGEAPYGQPPIMIDDFMNAEQIVKS 125 Query: 136 YPPFKKSILDRSLN---ISEVSCIPFTVGWYGETTT--RRELKASCFYRDGSV-NVFTRP 189 ++ +++ R L+ + V PF+ G + RR ++ +YR+ N + P Sbjct: 126 DEAWRVAVMKRGLSEKDLERVQVDPFSAGCFDRENENGRRLVRCVSYYRETLTDNGYAHP 185 Query: 190 IEGITVTIDVDSMQVIKYSDRFRK-PIPDKEGN-------DFRTKHRPFPFFCNVSDTGF 241 IEG+ +D+ +VI+ D R PIP + N + R+ +P F Sbjct: 186 IEGVMAVVDLLEKKVIELVDDGRIIPIPRAKHNYDTPSLGEPRSTLKPLSID-QPDGPSF 244 Query: 242 KILGNRVKWANWKFHVGFTARAGVTISTASVLDPRTKRFRRVMYRGHVSETFVPYMDPTY 301 I G V W NW F VGFT R G+ + S D ++ R ++YR V+E VPY DPT Sbjct: 245 TIDGWHVNWQNWNFRVGFTPREGLVLHQLSWDDGKSTR--PIIYRASVTEMCVPYSDPTT 302 Query: 302 EWYYRTFMDIGEFGFGRSAVNLQPLIDCPQNAAFLDGHVAGPDGTAQKMTNVMCVFEKNG 361 Y+++ D GE+G G+ A L+ DC + D A G M N +C+ E++ Sbjct: 303 NHYWKSAFDAGEYGLGKLANQLELGCDCLGTIRYFDIPSADDFGNPFVMKNAVCMHEED- 361 Query: 362 YGASFRHTEINVPGQVITSGEAEISLVVRMVATLGNYDYIVDWEFKKNGAIRVGVDLTGV 421 YG ++H E + LV+ AT+GNYDY W ++G I++ LTG+ Sbjct: 362 YGTLWKHYEFRTG---VFEMRRSRRLVISFFATVGNYDYGFYWYLYQDGTIQLECKLTGI 418 Query: 422 LEVKATS----YTSNDQITENVYGTLVAKNTIAVNHDHYLTYYLDLDVDGNGNSLVKAKL 477 ++ A + Y ITEN+ G H H+ + + VDG N++ + + Sbjct: 419 VQTSAVADGDTYPWGGMITENLGGP---------THQHFFNARMHMMVDGERNTVTEHEF 469 Query: 478 KTVRVTEVNKTSSRRKSYWTVVKETAKTEADG-RVRLGSDPVELLIVNPNKKTKIGNTVG 536 + E N + + K KTE++ R GS + NPN K +G G Sbjct: 470 VPRPMGENNPYGN----VFDTTKRVLKTESEAARNANGSTGRYWKVSNPNVKNAVGANPG 525 Query: 537 YRLIPEHLQATSLLTDDDYPELR--AGYTKYPVWVTAYDRSERWAGGFYSDRSRGDDGLA 594 Y+L+ + + L+ D+ ++R G+ VWVT +D +ER+A G Y ++ G DGL Sbjct: 526 YKLV---VNDSPLMLADERSKVRQRGGFATRHVWVTPFDPAERYASGDYPNQHSGGDGLP 582 Query: 595 VWSSRNREIENKDIVMWYNVGFHHIPYQEDFPVMPTLHGGFTLRPSNFFDNDP 647 + NR IEN+D+V+W++ G H+ EDFPVMP + GF L+P+NFF +P Sbjct: 583 RYIEANRNIENEDVVLWHSFGHTHVCKPEDFPVMPVEYAGFMLKPNNFFSANP 635 Lambda K H 0.320 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1204 Number of extensions: 60 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 650 Length of database: 661 Length adjustment: 38 Effective length of query: 612 Effective length of database: 623 Effective search space: 381276 Effective search space used: 381276 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory