Alignments for a candidate for etoh-dh-nad in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-nad in Burkholderia phytofirmans PsJN

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate BPHYT_RS21745 BPHYT_RS21745 choline dehydrogenase

Query= BRENDA::Q76HN6
         (526 letters)



>FitnessBrowser__BFirm:BPHYT_RS21745
          Length = 551

 Score =  419 bits (1077), Expect = e-121
 Identities = 237/528 (44%), Positives = 304/528 (57%), Gaps = 5/528 (0%)

Query: 3   FDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNWAF 62
           FD+++VGAGSAGCVLANRLS     +VCLLEAGP DR   IH P+G    +     NW F
Sbjct: 5   FDFVVVGAGSAGCVLANRLSEGGRYSVCLLEAGPADRFMWIHIPIGYGKTMFHPVYNWGF 64

Query: 63  KTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLPYF 122
            T P P +  R  Y PRG+ LGGSSSING+IY+RG +DD+++W  LGN GW + D LPYF
Sbjct: 65  YTDPDPNMHNRRLYWPRGRTLGGSSSINGLIYVRGQRDDYDNWARLGNRGWSWQDCLPYF 124

Query: 123 RKSEMHHGGSSEYHGGDGELYVSP-ANRHAASEAFVESALRAGHSYNPDFNGATQEGAGY 181
           ++ E +  G     G DG L+ S    RH   +AF+E++   G +   DFN   QEG GY
Sbjct: 125 KRLEHNELGEGPTRGVDGPLWASTIKQRHELVDAFIEASNSLGVASIDDFNTGDQEGVGY 184

Query: 182 YDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVHLR 241
           Y +T R G R STA A+LKP R R NL V T      I+  G +A GVQ    G    +R
Sbjct: 185 YQLTTRRGFRCSTAVAYLKPARQRQNLHVETDAMASKILFEGTRACGVQYRQHGELREVR 244

Query: 242 ARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDHADVVLCYKSN 301
           A +EVIL+AGA  SP LL LSG+G AA L   GI       GVG+NLQDH  + L Y+  
Sbjct: 245 ANREVILTAGALQSPQLLQLSGVGPAALLREFGIPVVANRAGVGENLQDHLQIRLIYEVT 304

Query: 302 DTSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDP-GLERPDIQLHSVIG 360
                   L       K    +A  R+GP+A    + G F +  P     PD Q H    
Sbjct: 305 KPITTNDQLRSWTGRAKMGLQWALMRSGPLAVGINQGGMFCRALPEESATPDTQFHFSTL 364

Query: 361 TVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLLKG 420
           + D     +H   G +  +C LRP+S G+V + S DPR+AP I PN+L  D D  T + G
Sbjct: 365 SADSAGGNVHDFPGCTYSICQLRPESRGAVRIRSADPREAPSIQPNYLDTDLDRRTTIAG 424

Query: 421 YRITRDIIAQTPMASFGLRDMY-SAGLHNDEQLIELLRKRTDTIYHPIGTCKMGQ--DEM 477
            R  R + A  PMA    R++   A    D++L+E  R+   TI+HP GT KMG   D +
Sbjct: 425 VRFARRVAAAQPMAGLMKREVRPGADAQTDDELLEFCREYGQTIFHPSGTAKMGPAGDPL 484

Query: 478 AVVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWIAH 525
           AVVD +LRV+G +GLRVVD SIMPTLV GNTN   +M+AE+A++ I H
Sbjct: 485 AVVDERLRVYGTQGLRVVDCSIMPTLVSGNTNVPIVMVAEKASDMILH 532


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 787
Number of extensions: 34
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 551
Length adjustment: 35
Effective length of query: 491
Effective length of database: 516
Effective search space:   253356
Effective search space used:   253356
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources