Alignments for a candidate for BPHYT_RS16930 in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for BPHYT_RS16930 in Burkholderia phytofirmans PsJN

Align Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale)
to candidate BPHYT_RS20740 BPHYT_RS20740 D-ribose transporter ATP binding protein

Query= uniprot:B2SYR5
         (512 letters)



>FitnessBrowser__BFirm:BPHYT_RS20740
          Length = 503

 Score =  404 bits (1038), Expect = e-117
 Identities = 221/496 (44%), Positives = 320/496 (64%), Gaps = 5/496 (1%)

Query: 1   MSATLRFDNIGKVFPGVRALDGVSFDVNVGQVHGLMGENGAGKSTLLKILGGEYQPDSGR 60
           M+  +    + K FPGVRAL  V F++  G+VH LMGENGAGKSTL+KIL G Y  D+G 
Sbjct: 1   MTPLISVKRLSKRFPGVRALHEVQFELVAGEVHALMGENGAGKSTLMKILAGVYTRDTGE 60

Query: 61  VMIDGNEVRFTSAASSIAAGIAVIHQELQYVPDLTVAENLLLGQLPNS-LG-WVNKREAK 118
           ++ +G  V F S   + A G+ +IHQELQ +  LT+A+N+ +G+ P   LG ++++ +  
Sbjct: 61  ILYEGQPVDFQSPREAQAVGVGIIHQELQLMNHLTIAQNMFIGREPRGRLGLFLDEDKLN 120

Query: 119 RFVRERLEAMGVALDPNAKLRKLSIAQRQMVEICKALLRNARVIALDEPTSSLSHRETEV 178
               + L  M V LDP A +  L++A++QMVEI KAL  ++RV+ +DEPTS+L+  E   
Sbjct: 121 AQAHDILARMHVTLDPRALVSSLTVARQQMVEIAKALSFDSRVLIMDEPTSALNDAEIAE 180

Query: 179 LFKLVRDLRADNRAMIYISHRMDEIYELCDACTIFRDGRKIASHPTLEGVTRDTIVSEMV 238
           LF+++RDL+     +IYISH+MDE+ ++ D  T+ RDG  +A+   ++  T   I+  MV
Sbjct: 181 LFRIIRDLKKRGVGIIYISHKMDELKQIADRVTVLRDGEYVAT-VAVKDTTVQAIIGMMV 239

Query: 239 GREISDIY-NYSARPLGEVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMH 297
           GR ++D   +      GEV      +    L +  SF +R+GEI+GF GL+GAGR+E+  
Sbjct: 240 GRTLTDAAPSQHIANQGEVALEVTRLNAGPLVRDVSFALRKGEILGFAGLMGAGRTEVAR 299

Query: 298 LVYGADHKKGGELLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCR 357
            V+GAD  + GE+++ G    +R+  +A+  GI    EDRK  G+    +V  NI +S  
Sbjct: 300 AVFGADPIESGEIVVKGVKATIRNPSDAVARGIGYLSEDRKRFGLATGMSVESNIVMSNL 359

Query: 358 RHYLRVGMFLDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLK 417
           R +L +  FL R +  +TA  FI LL I+TPS  Q++R LSGGNQQK ++++WL E D  
Sbjct: 360 RKFLSLNFFLRRTQIRKTAAHFINLLAIRTPSATQEVRLLSGGNQQKIVIAKWL-ERDCD 418

Query: 418 VVILDEPTRGIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISG 477
           V+  DEPTRGIDVGAK EIY ++  LA++G AIVMISSELPE+L +SDRIVVM +GRI+G
Sbjct: 419 VLFFDEPTRGIDVGAKSEIYKLLRSLADQGKAIVMISSELPEILRMSDRIVVMCEGRITG 478

Query: 478 ELTRKDATEQSVLSLA 493
           EL    AT++ ++ LA
Sbjct: 479 ELAAAGATQERIMQLA 494



 Score =  103 bits (258), Expect = 1e-26
 Identities = 72/259 (27%), Positives = 133/259 (51%), Gaps = 6/259 (2%)

Query: 252 PLGEVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYGADHKKGGELL 311
           PL  V+  +K   G        FE+  GE+    G  GAG+S LM ++ G   +  GE+L
Sbjct: 3   PLISVKRLSKRFPGVRALHEVQFELVAGEVHALMGENGAGKSTLMKILAGVYTRDTGEIL 62

Query: 312 LDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCRRHYLRVGMFLDRKK 371
            +G+P+  +S  EA   G+ +  ++ +   ++   T+++N+ I  R    R+G+FLD  K
Sbjct: 63  YEGQPVDFQSPREAQAVGVGIIHQELQ---LMNHLTIAQNMFIG-REPRGRLGLFLDEDK 118

Query: 372 EAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTRGIDVG 431
               A   +  + + T   R  +  L+   QQ   +++ L+  D +V+I+DEPT  ++  
Sbjct: 119 LNAQAHDILARMHV-TLDPRALVSSLTVARQQMVEIAKALSF-DSRVLIMDEPTSALNDA 176

Query: 432 AKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISGELTRKDATEQSVLS 491
              E++ +I  L +RG  I+ IS ++ E+  ++DR+ V+R G     +  KD T Q+++ 
Sbjct: 177 EIAELFRIIRDLKKRGVGIIYISHKMDELKQIADRVTVLRDGEYVATVAVKDTTVQAIIG 236

Query: 492 LALPQSSTALPGTQAAAQQ 510
           + + ++ T    +Q  A Q
Sbjct: 237 MMVGRTLTDAAPSQHIANQ 255


Lambda     K      H
   0.320    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 613
Number of extensions: 34
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 503
Length adjustment: 34
Effective length of query: 478
Effective length of database: 469
Effective search space:   224182
Effective search space used:   224182
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources