Aligments for a candidate for BPHYT_RS16930 in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Aligments for a candidate for BPHYT_RS16930 in Burkholderia phytofirmans PsJN

Align Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale)
to candidate BPHYT_RS27185 BPHYT_RS27185 D-ribose transporter ATP-binding protein

Query= uniprot:B2SYR5
         (512 letters)



>FitnessBrowser__BFirm:BPHYT_RS27185
          Length = 516

 Score =  401 bits (1030), Expect = e-116
 Identities = 217/494 (43%), Positives = 319/494 (64%), Gaps = 4/494 (0%)

Query: 5   LRFDNIGKVFPGVRALDGVSFDVNVGQVHGLMGENGAGKSTLLKILGGEYQPDSGRVMID 64
           L+   + K FPGV ALDG+  D+  G+VH + GENGAGKSTL+KI+ G+Y+ D G V   
Sbjct: 24  LQLKGVSKRFPGVVALDGIDLDLCAGEVHAVCGENGAGKSTLMKIISGQYRADEGVVRYR 83

Query: 65  GNEVRFTSAASSIAAGIAVIHQELQYVPDLTVAENLLLGQLPNSLGWVNKREAKRFVRER 124
           G  V+F+S + + AAGIA+IHQEL  VP L+VAEN+ L + P    +V+ R      +  
Sbjct: 84  GAPVQFSSTSDAQAAGIAIIHQELNLVPHLSVAENIYLAREPKRGPFVDYRTLNSNAQRC 143

Query: 125 LEAMGVALDPNAKLRKLSIAQRQMVEICKALLRNARVIALDEPTSSLSHRETEVLFKLVR 184
           L+ +G+ + P+  +  LS+AQ+QMVEI KAL  +ARV+ +DEPTSSL+  ET  LF+++R
Sbjct: 144 LQRIGLNVSPSTLVGALSLAQQQMVEIAKALSLDARVLIMDEPTSSLTESETVQLFRIIR 203

Query: 185 DLRADNRAMIYISHRMDEIYELCDACTIFRDGRKIASHPTLEGVTRDTIVSEMVGREISD 244
           +LRA   A++YISHR+DE+ E+ D  T+ RDGR IA+       T + IV+ MVGR + D
Sbjct: 204 ELRAGGVAILYISHRLDEMAEIVDRVTVLRDGRHIAT-SDFASTTVNEIVARMVGRPLDD 262

Query: 245 IY-NYSARPLGEVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYGAD 303
            Y    + P  ++    + ++   +  P SFE+R+GEI+GF GL+GAGR+E    ++GA+
Sbjct: 263 AYPPRQSTPSNQILLRVRDLQRTGVFGPLSFELRKGEILGFAGLMGAGRTETARAIFGAE 322

Query: 304 HKKGGELLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCRRHYLRV 363
               G + L  +P+ + S  EAIRHGI    EDRK++G+     VS NI ++  R     
Sbjct: 323 RPDSGSITLGDEPVTIGSPREAIRHGIAYLSEDRKKDGLALSMPVSANITLANVRAISSR 382

Query: 364 GMFLDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDE 423
           G FL   +E   A+R+++ L I+TP+ +Q  R LSGGNQQK ++S+WL     +++  DE
Sbjct: 383 G-FLRFSEETAIAERYVRELGIRTPTVKQIARNLSGGNQQKIVISKWLYRGS-RILFFDE 440

Query: 424 PTRGIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISGELTRKD 483
           PTRGIDVGAK+ IY ++ +LA  G  +V+ISSELPE+LG++DRI V  +GRI+  L  + 
Sbjct: 441 PTRGIDVGAKYAIYGLMDRLAADGVGVVLISSELPELLGMTDRIAVFHEGRITAVLETRQ 500

Query: 484 ATEQSVLSLALPQS 497
            +++ +L  A  +S
Sbjct: 501 TSQEEILHHASGRS 514



 Score = 75.5 bits (184), Expect = 4e-18
 Identities = 59/225 (26%), Positives = 108/225 (48%), Gaps = 8/225 (3%)

Query: 250 ARPLGEVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYGADHKKGGE 309
           +R + +++  +K   G         ++  GE+    G  GAG+S LM ++ G      G 
Sbjct: 20  SREILQLKGVSKRFPGVVALDGIDLDLCAGEVHAVCGENGAGKSTLMKIISGQYRADEGV 79

Query: 310 LLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCRRHYLRVGMFLDR 369
           +   G P++  S  +A   GI +     +E  +V   +V+ENI ++      + G F+D 
Sbjct: 80  VRYRGAPVQFSSTSDAQAAGIAII---HQELNLVPHLSVAENIYLA---REPKRGPFVDY 133

Query: 370 KKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTRGID 429
           +     A R ++ + +   S    +  LS   QQ   +++ L+  D +V+I+DEPT  + 
Sbjct: 134 RTLNSNAQRCLQRIGLNV-SPSTLVGALSLAQQQMVEIAKALS-LDARVLIMDEPTSSLT 191

Query: 430 VGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGR 474
                +++ +I +L   G AI+ IS  L E+  + DR+ V+R GR
Sbjct: 192 ESETVQLFRIIRELRAGGVAILYISHRLDEMAEIVDRVTVLRDGR 236


Lambda     K      H
   0.320    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 641
Number of extensions: 26
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 516
Length adjustment: 35
Effective length of query: 477
Effective length of database: 481
Effective search space:   229437
Effective search space used:   229437
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources