GapMind for catabolism of small carbon sources

 

Aligments for a candidate for PfGW456L13_1895 in Burkholderia phytofirmans PsJN

Align ABC transporter for D-Galactose and D-Glucose, permease component 1 (characterized)
to candidate BPHYT_RS29185 BPHYT_RS29185 sugar ABC transporter permease

Query= reanno::pseudo13_GW456_L13:PfGW456L13_1895
         (302 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS29185 BPHYT_RS29185 sugar ABC
           transporter permease
          Length = 314

 Score =  263 bits (673), Expect = 3e-75
 Identities = 121/275 (44%), Positives = 183/275 (66%)

Query: 23  LVLAPSMLIVLVGFYGYIIWTFILSFTNSSFMPSYKWVGLQQYMRLMDNDRWWVASKNLA 82
           L L P ++ V+  + G ++WT  +S +NS   PS  + G  QY RL +NDRW ++ +N+ 
Sbjct: 33  LALLPMVMTVVFAYLGTMVWTARVSLSNSRTFPSGDFAGFTQYARLFNNDRWLLSLQNIV 92

Query: 83  LFGGMFISISLVLGVFLAVLLDQRIRKEGFIRTVYLYPMALSMIVTGTAWKWLLNPGLGL 142
           ++G  FI   +V+G+ LA+ +DQR+  EG +RTV+LYP A+S + TG  W+W+LNP LG 
Sbjct: 93  IYGACFIVACMVIGLLLAIFIDQRVVAEGALRTVFLYPYAMSFVATGLVWQWILNPELGA 152

Query: 143 DKMLRDWGWEGFRLDWLVDQDRVVYCLVIAAVWQASGFVMAMFLAGLRGVDQSIIRAAQV 202
             +L   G+   R DW+VDQD V+Y +VIA VWQASG VMA+ LAGLRG+D  + +AA++
Sbjct: 153 QAVLHKLGFAHARFDWIVDQDWVIYTIVIATVWQASGLVMALLLAGLRGIDDELWKAARI 212

Query: 203 DGASLPTIYLKIVLPSLRPVFFSAFMILAHIAIKSFDLVAAMTAGGPGYSSDLPAMFMYS 262
           DG     +Y+ IV+P L P   +AF++L  + +K +D V AMT GGPG +S++PA F+  
Sbjct: 213 DGIPRWRVYVSIVVPMLGPSISTAFVLLFVMVVKLYDAVVAMTQGGPGTASEVPAKFIMD 272

Query: 263 FTFSRGQMGIGSASAMLMLGAVLTILVPYLYSELR 297
           + F R  +G+ SA+++++L  VL IL P+ Y+  R
Sbjct: 273 YLFGRANIGLASAASIVLLATVLAILAPFFYARSR 307


Lambda     K      H
   0.329    0.141    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 340
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 302
Length of database: 314
Length adjustment: 27
Effective length of query: 275
Effective length of database: 287
Effective search space:    78925
Effective search space used:    78925
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory