Alignments for a candidate for uxaA in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for uxaA in Burkholderia phytofirmans PsJN

Align Altronate dehydratase; EC 4.2.1.7; D-altronate hydro-lyase (uncharacterized)
to candidate BPHYT_RS28730 BPHYT_RS28730 galactarate dehydratase

Query= curated2:O34673
         (497 letters)



>FitnessBrowser__BFirm:BPHYT_RS28730
          Length = 503

 Score =  287 bits (735), Expect = 5e-82
 Identities = 167/492 (33%), Positives = 268/492 (54%), Gaps = 14/492 (2%)

Query: 5   IKIHKQDNVLLALRDIQKGERLHAYGVSIEVKDDIKRGHKIALQSIKENDSIVKYGFPIG 64
           ++++  D+V++AL  +  G  +    V+  V   I  GHK+A + I++ +++ +YG  IG
Sbjct: 1   MRLNPADDVVIALEQLMSGTVIDKEAVT--VSGLIPPGHKVATRDIRQGEAVHRYGQVIG 58

Query: 65  HASQDISIGEHIHVHNTKTNLSDIQLYSYTPRFDENPYSNENRTFKGFRRENGDAGVRNE 124
            ASQ I  G+H+H HN        + Y++         SN   TF+G  R +G    RN 
Sbjct: 59  FASQPIRAGQHVHTHNLAMG-DFARDYAFGQAARPTLPSNRPATFQGIVRADGRVATRNY 117

Query: 125 LWIVPTVGCVNGIAEKMLQRFVR-----ETGDIAPFDNVLVLKHQYGCS--QLGDDHENT 177
           + I+ +V C   +A  +   F R     E  +    D V+ L H  GC+    GD     
Sbjct: 118 IGILTSVNCSATVARAIADHFRRDIHPEELAEFPNVDGVIALTHGSGCAIDSEGDGLAIL 177

Query: 178 KQILLNAIRHPNAGGVLVLGLGCENNELARMKEAL---QDVNLKRVKFLESQSVTDEMEA 234
           ++ +     HPN   VLV+GLGCE N++ R+ E     +   L+     +S   T  +  
Sbjct: 178 QRTIGGYACHPNFASVLVVGLGCETNQIPRLLETQGLREHDRLRTFTIQDSGGTTSTIAK 237

Query: 235 GVALLKEIHEAAKGDKREDIPLSELKIGLKCGGSDGFSGITANPLLGRFSDYLIAQGGST 294
           G+ L++ +   A   +RE +  S L +GL+CGGSDG+SGITANP+LG   D L+  GG+ 
Sbjct: 238 GIELVRRMLPEANDVRREAVSASHLTVGLQCGGSDGYSGITANPVLGAAVDLLVRHGGTA 297

Query: 295 VLTEVPEMFGAETILMQRAANEEVFHKIVDLINDFKQYFIKHDQPVYENPSPGNKAGGIS 354
           +L+E PE++GAE +L +RAA  EV  K++D I  ++ Y  +H   +  NPS GNKAGG++
Sbjct: 298 ILSETPEIYGAEHLLTRRAATPEVGRKLLDRIRWWEAYANRHGAQMNNNPSAGNKAGGLT 357

Query: 355 TLEDKSLGCTQKAGISPVTDVLKYGEVLKTKGLTLLSAPGNDLIASSALAAAGCQIVLFT 414
           T+ +KSLG   KAG + +T+V +Y + +  +GL  +  PG D ++++   A G  ++ FT
Sbjct: 358 TVLEKSLGGIAKAGSTNLTEVYEYAQPVTARGLVFMDTPGYDPVSATGQVAGGANLICFT 417

Query: 415 TGRGTPFGTF-VPTVKVATNTELYEAKPHWIDFNAGLLAEDDVHEEYVLREFIHYMIEVA 473
           TGRG+ +G    P+VK+ATNT L++ +   +D N G + E     E +       ++EVA
Sbjct: 418 TGRGSAYGCAPTPSVKLATNTALWQRQSDDMDLNCGEVLEGRTTIEQLGGVLFELLLEVA 477

Query: 474 SGQLVNHEKNDF 485
           SG     E++ +
Sbjct: 478 SGNRTRSEQHGY 489


Lambda     K      H
   0.317    0.137    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 593
Number of extensions: 27
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 497
Length of database: 503
Length adjustment: 34
Effective length of query: 463
Effective length of database: 469
Effective search space:   217147
Effective search space used:   217147
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources