GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gntC in Burkholderia phytofirmans PsJN

Align ABC transporter substrate-binding protein (characterized, see rationale)
to candidate BPHYT_RS24175 BPHYT_RS24175 C4-dicarboxylate ABC transporter

Query= uniprot:A0A165IVH1
         (339 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS24175 BPHYT_RS24175
           C4-dicarboxylate ABC transporter
          Length = 330

 Score =  151 bits (382), Expect = 2e-41
 Identities = 95/287 (33%), Positives = 153/287 (53%), Gaps = 10/287 (3%)

Query: 5   RRTLLAALSVAAI-TCSFQAAAQDFKPRIIRFGYGLNEVSNQGRATKLFAEEVEKASGGK 63
           R +L+ A SV A+ T S QA       R+ R      +      A K   EE+ KA+GGK
Sbjct: 9   RVSLIVAASVLALSTVSAQA-------RVFRVSDVHGDTYPTNMAVKHMGEEINKATGGK 61

Query: 64  MKVRAIGAAALGSDVQMQQALIGGAQEMMVGSTATLVGITKEMAIWDTPFLFNNAKEADV 123
             V+  G +ALGS+      +  GA +M   + A    I  E  I   PFLF +      
Sbjct: 62  DSVKVFGNSALGSENDTIDQVRIGALDMARANGAAFNEIVPESMIPSLPFLFRDIDHFRK 121

Query: 124 VLDGPVGQKVMDKLQEKGLVGLVYWENGFRNLTNSKRPVNKLEDMDGIKLRVMQNNVFLD 183
           V+ GP GQK++D  + KG++ L ++E+G R++  +K+P++   DM G+K+RV  +++ +D
Sbjct: 122 VMYGPEGQKILDAFKAKGMIALTFYESGARSIY-TKKPIHTPADMKGLKVRVQPSDLMVD 180

Query: 184 SFKTLGANAVPLPFSELFTALETKTVDGQENPYNTILSSKFYEVQKYLTVTNHVYSPWIV 243
             + +G    P+PF+E++T L+T  VD  EN   +   +K +EV    + T H  +P ++
Sbjct: 181 EIRAMGGTPTPMPFAEVYTGLKTGLVDAAENNLPSYEETKHFEVAPVYSETQHSMTPEVL 240

Query: 244 LVSKKYWDGLSKAEQKVLLDAAKKSRD-FERQDTRAEADKALADLKG 289
           + SKK WD L+  EQ+++  AA  S   +++  T  EAD +    KG
Sbjct: 241 VFSKKVWDTLTPQEQEIIKKAAADSVPYYQKLWTAREADASKTVTKG 287


Lambda     K      H
   0.316    0.130    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 244
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 339
Length of database: 330
Length adjustment: 28
Effective length of query: 311
Effective length of database: 302
Effective search space:    93922
Effective search space used:    93922
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory