GapMind for catabolism of small carbon sources

 

Aligments for a candidate for lysP in Burkholderia phytofirmans PsJN

Align uncharacterized amino-acid permease C869.11 (characterized)
to candidate BPHYT_RS07280 BPHYT_RS07280 amino acid permease

Query= CharProtDB::CH_091412
         (580 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS07280 BPHYT_RS07280 amino acid
           permease
          Length = 466

 Score =  180 bits (456), Expect = 1e-49
 Identities = 121/404 (29%), Positives = 194/404 (48%), Gaps = 23/404 (5%)

Query: 73  LKRSLKSRHMQMISIGGAIGTGLYVGSGSSLADGGPASVIINYSLIGIMMFFIVYALGEM 132
           L+R L +  + MI+IGGAIGTGL++GSG ++   GP SV+++Y++  ++   ++  L EM
Sbjct: 18  LQRGLSTGQLSMIAIGGAIGTGLFLGSGFAIGFAGP-SVLVSYAIGALIALLLMGCLAEM 76

Query: 133 AVAYPVAGGFNTYATRFIDPAWGFAVSWNYFINYFVTFPLELTTCAITFRYW-TDINSAA 191
            VA+P +G F  YA  +I P  GF V + Y+ +       E+T  A+  +YW   +    
Sbjct: 77  TVAHPTSGSFGAYAEHYIAPWAGFLVRYAYWSSIVFAVGTEVTAIAVYMKYWFPAVPGWY 136

Query: 192 WISIFLVVIIIVNLFGVRAYGEVEFILSTVKVVATFGFIILAIIINCGGVPTDHRGYIGG 251
           WI  F   +I +N   V+ +G VE++ S +K+VA  GFI+L   +   G P D       
Sbjct: 137 WIVGFSAALIGINSVSVKVFGAVEYVFSMLKIVAIVGFILLGAYV-VFGAPADSTIGFAN 195

Query: 252 SIIKHKPFRHGFKGFCSVFTTAAFSFSGTEVIGLAAAEVDNPQKALPHAVKQVFWRIAIF 311
                  F  G  G       + FS+   E+I +AA E  +PQKA+  A +   +R+  F
Sbjct: 196 YTSHGGFFPKGVWGMWVAVIVSIFSYLSIEMIAVAAGEARDPQKAITRAFRATMFRLVFF 255

Query: 312 YVVSLILIGLLISPDDPNLMGNGSTSVSPFVLAIKEANIKGLPSVFNAVIIISVVSVTNS 371
           Y+++L L+ L I P          T  SPFV  +   ++ G   V N VI+++ +S  NS
Sbjct: 256 YLLTLALM-LAIVP-----WNAAGTDESPFVRVMAATHVPGAAGVINFVILVAALSAMNS 309

Query: 372 STYTAGRTLHGMANLKQAPSFFKYTDRLGRPLLAM-IVVLLFGFFAYINEADKNGNDVSD 430
             Y   R +  ++    AP      +  G P+ A+ +  +       +N          D
Sbjct: 310 QLYITTRMMFSLSRAGYAPRKLGALNGKGVPVAALWLSTIGIALATVLNVV------YPD 363

Query: 431 TVFNWLLALSGLSNFFTWGSICLCHIIFR-------LAFKKQGH 467
             F  ++++S     FTW  I + H  FR       LAF+  G+
Sbjct: 364 ASFVLMMSVSMFGAMFTWLMIFVTHFFFRHRHQGAPLAFRMWGY 407


Lambda     K      H
   0.325    0.141    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 688
Number of extensions: 44
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 580
Length of database: 466
Length adjustment: 35
Effective length of query: 545
Effective length of database: 431
Effective search space:   234895
Effective search space used:   234895
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory