Align ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized)
to candidate BPHYT_RS22780 BPHYT_RS22780 sugar ABC transporter permease
Query= reanno::Smeli:SMc03063 (380 letters) >FitnessBrowser__BFirm:BPHYT_RS22780 Length = 283 Score = 142 bits (357), Expect = 1e-38 Identities = 86/235 (36%), Positives = 131/235 (55%), Gaps = 7/235 (2%) Query: 146 PPRFTL-DNYAEVLSAAGIGRSFLNSLTVAVPSTVIPILIAAFAAYALAWMPFPGRAVLL 204 P F + DNY E L+ + + F NS+ + VP+ V I +AA A +ALA F G + L Sbjct: 55 PKHFAMFDNYREALTTSPMLHYFWNSVLITVPAVVGSIALAAMAGFALAIYRFRGNSTLF 114 Query: 205 AVVVGLLVVPLQMSLIPLLQLYNGVGAFFGVSAKTYMGIWLAHTGFGLPLAIYLLRNYMA 264 A V VP+Q+ +IP+ L +G F VSA + L H F LRN++ Sbjct: 115 ATFVAGNFVPVQVLMIPVRDLSLQLGLFNTVSA-----LILFHVSFQTGFCALFLRNFIK 169 Query: 265 GLPREIMESARVDGASDFDIFVKIILPLSFPALASFAIFQFLWTWNDLLVAIVFLGAGDD 324 LP E++E+AR++GA+++ +F KI+LPL PALA+ AI F + WND A+ L GDD Sbjct: 170 QLPFELVEAARIEGANEWTVFFKIVLPLIRPALAALAILVFTFVWNDYFWALC-LTQGDD 228 Query: 325 KLVLTGRLVNLLGSRGGNWEILTASAFITIVVPLIVFFALQRYLVRGLLAGSVKG 379 +T + L G W +++A + + + + +FFA+Q++ V GL G+ KG Sbjct: 229 AAPITVGVAALKGQWTTAWNLVSAGSILAALPSVAMFFAMQKHFVAGLTFGATKG 283 Lambda K H 0.324 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 274 Number of extensions: 13 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 380 Length of database: 283 Length adjustment: 28 Effective length of query: 352 Effective length of database: 255 Effective search space: 89760 Effective search space used: 89760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory