Alignments for a candidate for HSERO_RS03640 in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS03640 in Burkholderia phytofirmans PsJN

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate BPHYT_RS19720 BPHYT_RS19720 arabinose ABC transporter ATP-binding protein

Query= uniprot:D8IZC7
         (521 letters)



>FitnessBrowser__BFirm:BPHYT_RS19720
          Length = 515

 Score =  405 bits (1042), Expect = e-117
 Identities = 220/495 (44%), Positives = 317/495 (64%), Gaps = 8/495 (1%)

Query: 5   PLLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEIL 64
           P L++ GI   F   LAL  + L +R GE+H LMGENGAGKSTL+KVLSGV+ P  G + 
Sbjct: 24  PYLRLDGITVRFPGVLALDQVSLEVRRGEVHGLMGENGAGKSTLLKVLSGVNQPAAGTLS 83

Query: 65  LDGRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRT 124
           LDG         A+ AAG+ +IYQEL + P ++VA N+ +G+ L  R G++D  A+ +R 
Sbjct: 84  LDGVEQQFITTKAAIAAGVAIIYQELHLVPELTVAENLMLGA-LPNRFGILDEKALVARA 142

Query: 125 DAVLRQLGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFN 184
              L +LG     S     LSI ++Q +EI +AL+  +R++  DEPT++LS RET QLF 
Sbjct: 143 VRELERLGEKIDPSQQVKNLSIGQRQMIEIGKALMRDARVIAFDEPTSSLSSRETTQLFR 202

Query: 185 VVRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDE-IDSERIVQMMVGRS 243
           ++R L+ EG AIIY++HRM EVY L DRVTV RDG  +      E +D +R++  MVGRS
Sbjct: 203 IIRALKAEGRAIIYVTHRMDEVYELCDRVTVFRDGRRIDTFEAGEGLDRDRLISCMVGRS 262

Query: 244 LSEFYQHQRIAPADAAQLPTVMQVRALAGGKIR-PASFDVRAGEVLGFAGLVGAGRTELA 302
           +++ Y ++     D       + V+ + G  +R PASF  R GE++GF GLVGAGR+EL 
Sbjct: 263 IADVYGYRSRDLGDVQ-----LDVKEMMGRGLREPASFTARKGEIVGFFGLVGAGRSELM 317

Query: 303 RLLFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATMNV 362
           +L++GA     G+I L+G+ V    PR A+RAG+A  PEDRK +G+    +V+ N  ++ 
Sbjct: 318 KLIYGAVKPDAGEIALKGKRVRFATPRDAVRAGVALCPEDRKQEGIVSIASVSDNLNISC 377

Query: 363 ASRHTRLGLVRSRSLGGVARAAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKV 422
               +R  ++  R     A+  I +L +K  + +TP+G LSGGNQQKV+L+RWL     V
Sbjct: 378 RRHFSRFNVLNGRKEAQTAKEFIGKLAIKTRNGDTPIGTLSGGNQQKVILSRWLAEDIDV 437

Query: 423 LILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGE 482
            ++DEPTRG+D+ A+SEIY L++ LA  G  V+V+SS+L EVIG+ DRV+VM+EG I G+
Sbjct: 438 FLMDEPTRGIDVGARSEIYGLLYGLAEAGRTVIVVSSDLAEVIGVADRVIVMKEGRIVGD 497

Query: 483 LAGAAITQENIMRLA 497
           L  A  T + +++LA
Sbjct: 498 LPKAQATPDALIKLA 512



 Score = 92.4 bits (228), Expect = 3e-23
 Identities = 66/232 (28%), Positives = 112/232 (48%), Gaps = 29/232 (12%)

Query: 28  TIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVALRDPGASRAAGINLIY 87
           T R GEI    G  GAG+S LMK++ G   PD GEI L G+ V    P  +  AG+ L  
Sbjct: 296 TARKGEIVGFFGLVGAGRSELMKLIYGAVKPDAGEIALKGKRVRFATPRDAVRAGVALCP 355

Query: 88  QE---------LAVAPNISVAA-------NVFMG-SELRTRLGLIDHAAMRSRTDAVLRQ 130
           ++          +V+ N++++        NV  G  E +T    I   A+++R       
Sbjct: 356 EDRKQEGIVSIASVSDNLNISCRRHFSRFNVLNGRKEAQTAKEFIGKLAIKTRN------ 409

Query: 131 LGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLR 190
                      G LS   QQ+V ++R L     + +MDEPT  +      +++ ++  L 
Sbjct: 410 ------GDTPIGTLSGGNQQKVILSRWLAEDIDVFLMDEPTRGIDVGARSEIYGLLYGLA 463

Query: 191 DEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGR 242
           + G  +I +S  +AEV  +ADRV V+++G  VG+L + +   + ++++ + R
Sbjct: 464 EAGRTVIVVSSDLAEVIGVADRVIVMKEGRIVGDLPKAQATPDALIKLALPR 515


Lambda     K      H
   0.320    0.135    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 733
Number of extensions: 35
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 521
Length of database: 515
Length adjustment: 35
Effective length of query: 486
Effective length of database: 480
Effective search space:   233280
Effective search space used:   233280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources