Aligments for a candidate for pad-dh in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Aligments for a candidate for pad-dh in Burkholderia phytofirmans PsJN

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate BPHYT_RS29875 BPHYT_RS29875 betaine-aldehyde dehydrogenase

Query= BRENDA::P05091
         (517 letters)



>FitnessBrowser__BFirm:BPHYT_RS29875
          Length = 483

 Score =  422 bits (1084), Expect = e-122
 Identities = 217/459 (47%), Positives = 294/459 (64%), Gaps = 6/459 (1%)

Query: 57  VNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDR 116
           +NP+T E I  VA+G   DVD AV+AARAA ++   W  +  + R R+L RLA L+  + 
Sbjct: 30  INPATEEPIALVAQGSAADVDTAVRAARAALKV---WNGIRTAERARILMRLAGLMRANL 86

Query: 117 TYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPV 176
             LAALE+LD GKP       D+   +  L YYAGW DK +G+ +P+  D  +YT  EPV
Sbjct: 87  EELAALESLDAGKPIAAVMRQDIPAAIDTLEYYAGWCDKINGQVVPVRPDALTYTLREPV 146

Query: 177 GVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVN 236
           GV   I+PWNFPL++  WK+ PALA G  +++K AE TPLTAL +  L  EAG PPGV+N
Sbjct: 147 GVVAAIVPWNFPLMIGMWKIAPALACGCTLIVKPAEITPLTALRIGELALEAGVPPGVLN 206

Query: 237 IVPGFGPTAGAAIASHEDVDKVAFTGSTEIGR-VIQVAAGSSNLKRVTLELGGKSPNIIM 295
           IV G G   G A+ +H  VDKV FTGS  +GR ++Q AAG  N KRVTLELGGKS N+I 
Sbjct: 207 IVTGKGRVVGDALVAHPGVDKVTFTGSPSVGRGILQGAAG--NFKRVTLELGGKSANLIF 264

Query: 296 SDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSK 355
            DA++D AV  A   +FFN GQ C AGSR     D+YDE VER  ARAKS  VG+P   +
Sbjct: 265 PDANLDNAVRAAASGIFFNTGQVCSAGSRILAHRDVYDEVVERLAARAKSIKVGDPSSRE 324

Query: 356 TEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKE 415
           T  GP +   Q K +LGY+ TG+ EGA L+ GG    +RG+F++PTVF +V+  M I++E
Sbjct: 325 TSMGPLISAAQMKTVLGYVETGRAEGASLVTGGARVGERGFFVEPTVFANVEHEMRISQE 384

Query: 416 EIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDV 475
           EIFGPV  +++F    + +  AN + Y LAA V++ D+ + + +++ L+AGTVW+N Y  
Sbjct: 385 EIFGPVASVIRFNDEADAIRIANGTLYSLAAGVWSADIGRVHRVARDLRAGTVWINTYGY 444

Query: 476 FGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQ 514
              + P+GG   SG GRE G+  ++ +TE K V + + Q
Sbjct: 445 TDVRLPWGGSGDSGFGREHGDVAIENFTEPKAVWLAIDQ 483


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 651
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 483
Length adjustment: 34
Effective length of query: 483
Effective length of database: 449
Effective search space:   216867
Effective search space used:   216867
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources