GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natA in Burkholderia phytofirmans PsJN

Align NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized)
to candidate BPHYT_RS31750 BPHYT_RS31750 ABC transporter

Query= TCDB::Q55164
         (267 letters)



>FitnessBrowser__BFirm:BPHYT_RS31750
          Length = 258

 Score =  188 bits (478), Expect = 9e-53
 Identities = 100/247 (40%), Positives = 154/247 (62%)

Query: 19  LLAQGLSKSFGGLRAVDHADIVVKEGSITGLIGPNGAGKTTLFNLLSNFIRPDQGEVLFN 78
           L  +G+SK FGGL+A+    + + EGSI GLIGPNGAGKTT FN+++    PD GE   +
Sbjct: 8   LSVKGVSKRFGGLQALSDVGLQIDEGSIYGLIGPNGAGKTTFFNVITGLYSPDSGEFELD 67

Query: 79  GDSIGQLAPHQIALRGSVRTFQVAKVLSRLTVLENMLLADQHQTGEKFLPRLINFRRVQK 138
                  A H++A  G  RTFQ  ++   +T LEN+++    +T +  +  ++     ++
Sbjct: 68  NKPYTPTAVHKVAKTGIARTFQNIRLFGGMTALENVMVGQHVRTKQGLIGAVLQTLSERR 127

Query: 139 EERANREKAMAMLESVGLGAKAQDYAGALSGGQRKLLEMARALMSNPKLILLDEPAAGVN 198
           EERA RE+A+ +L+ VG+   A   A  LS G ++ LE+ARAL ++PKL+ LDEPAAG+N
Sbjct: 128 EERAIRERAIELLDYVGIAQYADYTARNLSYGHQRRLEIARALATDPKLLALDEPAAGMN 187

Query: 199 PTLIGQICEHIVNWNRQGITFLVIEHNMDVIMTLCHHVWVLAEGRNLADGTPEQIQSDPR 258
            T   ++   +      G T L+IEH++ ++M +C+ + VL  G+ +A+G P  +Q DP+
Sbjct: 188 ATEKLELTGLLKQIRADGRTILLIEHDVKLMMGVCNGMTVLDYGKVIAEGLPRDVQRDPK 247

Query: 259 VLEAYLG 265
           V+EAYLG
Sbjct: 248 VIEAYLG 254


Lambda     K      H
   0.319    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 206
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 267
Length of database: 258
Length adjustment: 25
Effective length of query: 242
Effective length of database: 233
Effective search space:    56386
Effective search space used:    56386
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory