Alignments for a candidate for actP in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Burkholderia phytofirmans PsJN

Align actP-like component of D-alanine uptake system (characterized)
to candidate BPHYT_RS07010 BPHYT_RS07010 sodium:solute symporter

Query= reanno::psRCH2:GFF346
         (589 letters)



>FitnessBrowser__BFirm:BPHYT_RS07010
          Length = 672

 Score =  197 bits (502), Expect = 9e-55
 Identities = 122/347 (35%), Positives = 179/347 (51%), Gaps = 36/347 (10%)

Query: 239 ADVDNKL---NMFLFTLSLMIGTAGLPHVIIRFFTVPKVADARWSAGWTLVFIALLYLTA 295
           +D D ++   N     L L +GTA LPH++ R+ T   VA AR S GWTL F+AL YLT 
Sbjct: 357 SDEDRRIHERNFLSLLLCLSLGTASLPHILTRYNTTTSVASARRSVGWTLFFVALFYLTV 416

Query: 296 PAVASMARLNLVNTIYPEGPQAEAIRYEDRPEWVQTWERT--GLIKWEDKNADGRVQMYN 353
           P +A + +  ++  +           + D P+W+  W +    LI   D N DG V+   
Sbjct: 417 PVLAVLIKYEILTNLVGH-------HFSDLPQWLMQWRKVEPSLISLADTNGDGIVR--- 466

Query: 354 DANAKFTPTATERGWNGNELTVNNDIIVLANPEIANLPGWVIGLIAAGAIAAALSTAAGL 413
                         W+  E+ +  D++VLA PEIA LP  + GLIAAGA+AAALSTA GL
Sbjct: 467 --------------WS--EIQMQPDMVVLAAPEIAGLPYVMSGLIAAGALAAALSTADGL 510

Query: 414 LLAISSAISHDLIKTLINPKISEKNEMLAARLSMTAAILLATWLGLNPPGFAAQVVALAF 473
           LL I++A+SHD+   +++P  S +  +  +++ +    L A+++     G    +V  AF
Sbjct: 511 LLTIANALSHDVYYHMVDPNASSQRRVTISKILLLGVALFASYVASLNTGNILFLVGAAF 570

Query: 474 GLAAASLFPALMMGIFSKRVNSKGAVAGMLVGVISTAVYIFLYLGWFFIPGTASIPNTPD 533
            LAA+SLFP L++G+F KR    GAVAGM+ G++    YI     +F       +     
Sbjct: 571 SLAASSLFPVLVLGVFWKRTTRLGAVAGMVAGLVVCIYYIVSTYPFF-----TQMTGFAG 625

Query: 534 QWWMGISPQAFGAVGAMLNFAVAYAVSMATEAPPQEIQDLVESVRTP 580
             W GI P + G  G    F VA  VS+    P    + LV+ +R P
Sbjct: 626 PRWFGIEPISSGVFGVPAGFLVAIGVSLIDRKPDAYTRALVDYIRHP 672



 Score =  181 bits (458), Expect = 1e-49
 Identities = 92/204 (45%), Positives = 125/204 (61%)

Query: 5   WINMLFVGASFLLYIGIAVWARAGSTKEFYVAGGGVHPVTNGMATAADWMSAASFISMAG 64
           WI  +F+     +Y  I + +R     E+YVAG  V    NGMATAADW+SAASFI +AG
Sbjct: 37  WIGYVFLFVPIAVYAVIGLLSRTSDLVEYYVAGRRVPSAFNGMATAADWLSAASFIGLAG 96

Query: 65  LIASGGYATSVYLMGWTGGYVLLAMLLAPYLRKFGKFTVPDFIGDRFYSRGARLTAVVCL 124
            I + GY    YLMGWTGGY L+A LLAPY+RK  ++T+PDF+G RF S   R  A +  
Sbjct: 97  SIYATGYDGLAYLMGWTGGYCLVAFLLAPYVRKLARYTIPDFLGTRFSSNAVRGLAALAA 156

Query: 125 ILISVTYVIGQMAGAGVAFSRFLEVSNSAGIWIAAAIVFAYAVFGGMKGITYTQVAQYIV 184
           +L S  Y++ Q+ G G+  +RF+ V  + GI+   A +   +  GGM+ +T+TQVAQYI+
Sbjct: 157 VLCSFVYLVAQIQGVGLIATRFIGVDFAVGIFCGLAGILVCSFLGGMRAVTWTQVAQYII 216

Query: 185 LIIAYTIPAVFIAMQLTGNPIPMF 208
           LI A  IP   IA +     +P F
Sbjct: 217 LIAAILIPVSMIAHKDGLGWVPQF 240


Lambda     K      H
   0.324    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 974
Number of extensions: 50
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 589
Length of database: 672
Length adjustment: 38
Effective length of query: 551
Effective length of database: 634
Effective search space:   349334
Effective search space used:   349334
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources