Alignments for a candidate for rhaT' in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Burkholderia phytofirmans PsJN

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate BPHYT_RS20740 BPHYT_RS20740 D-ribose transporter ATP binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>FitnessBrowser__BFirm:BPHYT_RS20740
          Length = 503

 Score =  372 bits (954), Expect = e-107
 Identities = 212/494 (42%), Positives = 312/494 (63%), Gaps = 6/494 (1%)

Query: 20  ILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKSTLVKILTGIYRPNEGEILV 79
           ++ ++ +S+ FPGV+AL  V   L  G V AL+GENGAGKSTL+KIL G+Y  + GEIL 
Sbjct: 4   LISVKRLSKRFPGVRALHEVQFELVAGEVHALMGENGAGKSTLMKILAGVYTRDTGEILY 63

Query: 80  DGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGHAPRTRFRT-IDWQTMNSRS 138
           +G+P  F S + A   GV  IHQE  L + LT+A+N+F+G  PR R    +D   +N+++
Sbjct: 64  EGQPVDFQSPREAQAVGVGIIHQELQLMNHLTIAQNMFIGREPRGRLGLFLDEDKLNAQA 123

Query: 139 KALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVIMDEPTAALSRKEIDDLFR 198
             +L  +   +DP   +  L++A++ +V IA+ALS ++R++IMDEPT+AL+  EI +LFR
Sbjct: 124 HDILARMHVTLDPRALVSSLTVARQQMVEIAKALSFDSRVLIMDEPTSALNDAEIAELFR 183

Query: 199 IVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRGVSRK-TPQDEIVRMMVGR 257
           I+R LK++G  I++ISHK DEL +IAD   V   R    V  V+ K T    I+ MMVGR
Sbjct: 184 IIRDLKKRGVGIIYISHKMDELKQIADRVTVL--RDGEYVATVAVKDTTVQAIIGMMVGR 241

Query: 258 DVENVFPKIDVAIGGPV-LEIRNYSHRTEFRDISFTLRKGEILGVYGLIGAGRSELSQSL 316
            + +  P   +A  G V LE+   +     RD+SF LRKGEILG  GL+GAGR+E+++++
Sbjct: 242 TLTDAAPSQHIANQGEVALEVTRLNAGPLVRDVSFALRKGEILGFAGLMGAGRTEVARAV 301

Query: 317 FGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLAR 376
           FG     SG++V++G + TI +P DA+  GI Y+ E+R R GLA  M +  N+ + +L +
Sbjct: 302 FGADPIESGEIVVKGVKATIRNPSDAVARGIGYLSEDRKRFGLATGMSVESNIVMSNLRK 361

Query: 377 -TSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVII 435
             S   FLR       A  +   L +R  + +  V  LSGGNQQK+VI KWL     V+ 
Sbjct: 362 FLSLNFFLRRTQIRKTAAHFINLLAIRTPSATQEVRLLSGGNQQKIVIAKWLERDCDVLF 421

Query: 436 LDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAGIFE 495
            DEPT+GID+G+K+ ++  +  LA +G +I+M+SSELPEI+ MSDR++VM EG   G   
Sbjct: 422 FDEPTRGIDVGAKSEIYKLLRSLADQGKAIVMISSELPEILRMSDRIVVMCEGRITGELA 481

Query: 496 RAELSPEALVRAAT 509
            A  + E +++ AT
Sbjct: 482 AAGATQERIMQLAT 495



 Score = 83.6 bits (205), Expect = 2e-20
 Identities = 70/248 (28%), Positives = 119/248 (47%), Gaps = 20/248 (8%)

Query: 273 PVLEIRNYSHR----TEFRDISFTLRKGEILGVYGLIGAGRSELSQSLFGITKPLSGKMV 328
           P++ ++  S R        ++ F L  GE+  + G  GAG+S L + L G+    +G+++
Sbjct: 3   PLISVKRLSKRFPGVRALHEVQFELVAGEVHALMGENGAGKSTLMKILAGVYTRDTGEIL 62

Query: 329 LEGQEITIHSPQDAIRAGIVYVPEERGRHGLALPMPIFQNMTLPSLARTSRRGF------ 382
            EGQ +   SP++A   G+  + +E     L   + I QNM +    R  R G       
Sbjct: 63  YEGQPVDFQSPREAQAVGVGIIHQELQ---LMNHLTIAQNMFIGREPR-GRLGLFLDEDK 118

Query: 383 LRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQKVVIGKWLATAPKVIILDEPTKG 442
           L A     LAR +   LD RA      V +L+   QQ V I K L+   +V+I+DEPT  
Sbjct: 119 LNAQAHDILARMHVT-LDPRAL-----VSSLTVARQQMVEIAKALSFDSRVLIMDEPTSA 172

Query: 443 IDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSDRVLVMKEGLSAGIFERAELSPE 502
           ++    A +   I +L   G+ II +S ++ E+  ++DRV V+++G         + + +
Sbjct: 173 LNDAEIAELFRIIRDLKKRGVGIIYISHKMDELKQIADRVTVLRDGEYVATVAVKDTTVQ 232

Query: 503 ALVRAATG 510
           A++    G
Sbjct: 233 AIIGMMVG 240


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 561
Number of extensions: 28
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 503
Length adjustment: 34
Effective length of query: 478
Effective length of database: 469
Effective search space:   224182
Effective search space used:   224182
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources