Alignments for a candidate for rbsA in Burkholderia phytofirmans PsJN

GapMind for catabolism of small carbon sources

Alignments for a candidate for rbsA in Burkholderia phytofirmans PsJN

Align Ribose ABC transporter ATPase; SubName: Full=Sugar ABC transporter ATP-binding protein; SubName: Full=Sugar ABC transporter ATPase (characterized, see rationale)
to candidate BPHYT_RS20685 BPHYT_RS20685 ribose ABC transporter ATPase

Query= uniprot:A0A1N7TZ92
         (517 letters)



>FitnessBrowser__BFirm:BPHYT_RS20685
          Length = 537

 Score =  714 bits (1843), Expect = 0.0
 Identities = 375/520 (72%), Positives = 435/520 (83%), Gaps = 7/520 (1%)

Query: 1   MSSSAPNAVLSVSGIGKTYAQPVLSDITLTLNRGEVLALTGENGAGKSTLSKIIGGLVTP 60
           ++ +AP  VL V GIGKTYA+PVL+D++L L+ GEVLALTGENGAGKSTLSKIIGGLVTP
Sbjct: 8   LADAAP--VLRVHGIGKTYAEPVLADVSLDLHAGEVLALTGENGAGKSTLSKIIGGLVTP 65

Query: 61  TTGHMQFNGQDFRPGSRTQAEELGVRMVMQELNLLPTLTVAENLFLDNLPSH--CGWISR 118
           TTG M   G  + P SR  AE LGVRMVMQELNLLPTL+VAENLFL+ LP     GWI R
Sbjct: 66  TTGSMTLAGAAYTPASRKDAEALGVRMVMQELNLLPTLSVAENLFLNRLPQRGIFGWIDR 125

Query: 119 KQLRKAAIEAMAQVGLDAIDPDTLVGSLGIGHQQMVEIARNLIGDCHVLILDEPTAMLTA 178
            +LR+ A  AMAQVGLDAIDPDTLVG+LGIGHQQMVEIARNLIGDC VLILDEPTAMLTA
Sbjct: 126 AKLREDARHAMAQVGLDAIDPDTLVGTLGIGHQQMVEIARNLIGDCRVLILDEPTAMLTA 185

Query: 179 REVEMLFEQITRLQARGVAIIYISHRLEELARVAQRIAVLRDGKLVCVEPMANYNSEQLV 238
           REV++LFEQ+ RL+ARGVA++YISHRLEEL R+A+R AVLRDG+LV V+ MAN  +++LV
Sbjct: 186 REVDLLFEQVERLKARGVALVYISHRLEELKRIARRAAVLRDGRLVHVDEMANLTTDRLV 245

Query: 239 TLMVGRELGEHIDLGPRTIGGPALTVKGLTRSDKVRDVSFEVRAGEIYGISGLIGAGRTE 298
           TLMVGR++G+ IDLG R IG     V G+TR   VRDVSFEV+AGEI+GISGLIGAGRTE
Sbjct: 246 TLMVGRDIGDRIDLGERRIGEMTFKVSGMTREPVVRDVSFEVKAGEIFGISGLIGAGRTE 305

Query: 299 LLRLIFGADLADSGTVAL---GSPAQVVSIRSPVDAVGHGIALITEDRKGEGLLLTQSIS 355
           L+RLI+GAD AD+GTV++   G   + V+I SP DAV HGIALITEDRKGEGLLLTQ I+
Sbjct: 306 LMRLIYGADRADAGTVSIVREGGALEPVTIASPSDAVKHGIALITEDRKGEGLLLTQPIA 365

Query: 356 ANIALGNMPEISGGGVVNSRDETALAKRQIDAMRIRSSSPAQLVSELSGGNQQKVVIGRW 415
           ANI+LG++  +S  GVV+ R E +LA+RQIDAMRIRSS PAQ VSELSGGNQQKVVIGRW
Sbjct: 366 ANISLGHLRSVSSKGVVDGRREASLARRQIDAMRIRSSGPAQPVSELSGGNQQKVVIGRW 425

Query: 416 LERDCSVMLFDEPTRGIDVGAKFDIYALLGELTRQGKALVVVSSDLRELMLICDRIGVLS 475
           L RDC+V+LFDEPTRGIDVGAKFDIYAL+G L R+G+ALVVVSSDLRELM ICDRI V+S
Sbjct: 426 LARDCTVLLFDEPTRGIDVGAKFDIYALMGALAREGRALVVVSSDLRELMAICDRIAVMS 485

Query: 476 AGRLIETFERDSWTQDELLAAAFAGYQKRDALLNDAVLRD 515
           AGR+   FER +WTQD LLAAAFAGY KRD +L++ +  D
Sbjct: 486 AGRMTGLFERGNWTQDALLAAAFAGYAKRDDMLHEPIEPD 525


Lambda     K      H
   0.319    0.136    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 915
Number of extensions: 41
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 537
Length adjustment: 35
Effective length of query: 482
Effective length of database: 502
Effective search space:   241964
Effective search space used:   241964
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources