Align α,α-trehalase (MSMEG_4535;MSMEG4528) (EC 3.2.1.28) (characterized)
to candidate BPHYT_RS06100 BPHYT_RS06100 glucoamylase
Query= CAZy::ABK72415.1 (668 letters) >FitnessBrowser__BFirm:BPHYT_RS06100 Length = 626 Score = 367 bits (943), Expect = e-106 Identities = 230/617 (37%), Positives = 328/617 (53%), Gaps = 35/617 (5%) Query: 42 IADYGFLSDCETTCLISSAGSVEWLCVPRPDSPSVFGAILDRGA-GHFRLGPYGVSVPA- 99 I DY + D T L+S GSV+WLC PR DS + F A+L G + + P + P Sbjct: 5 IEDYALIGDGHTAALVSRDGSVDWLCWPRFDSGACFAALLGTPENGRWLIAPVSDTPPTI 64 Query: 100 ARRYLPGSLILETTWQTHTGWLIVRDALVMGP-WHDIDTRSRTHRRTPMDWDAEHILLRT 158 RRY +LILET ++T G + + D + G W + ++R Sbjct: 65 TRRYRGETLILETDFETPEGAITLIDFMPPGNGWSE--------------------MVRL 104 Query: 159 VRCVSGTVELVMSCEPAFDYHRVSATWEYSGPAYGEAIARASRNPDSHPTLRLTTNLRIG 218 V GTV + M FDY S W ++ +A PD T L T + + Sbjct: 105 VVGRRGTVRMKMELVLRFDYG-FSVPWV--DRLTHDSGIKAIVGPD---TAVLRTPVELR 158 Query: 219 IEGREARARTRLTEGDNVFVALSWS-KHPAPQTYEEAADKMWKTSEAWRQWINVGDFPDH 277 E + A ++EG+ V +L+++ H + + +T W +W + + Sbjct: 159 GEDMKTVAEFTVSEGERVPFSLAYAASHLRIPPARDPHTSLARTENHWLEW-SARSTVEG 217 Query: 278 PWRAYLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWIRDSTFALWGLY 337 W ++RS +TLK L Y PTG ++AAPTTSLPE G RNWDYRY W+RD+T L + Sbjct: 218 RWAEPIRRSLITLKALAYEPTGGIVAAPTTSLPEQLGGTRNWDYRYCWLRDATITLLAMM 277 Query: 338 TLGLDREADDFFSFIADVSGANNGERHPLQVMYGVGGERSLVEEELHHLSGYDNSRPVRI 397 G EA + S++ V G LQ+MYG+ GER L E E+ L GY +++PVRI Sbjct: 278 RGGYYDEARAWRSWLGRVMA---GAPDQLQIMYGIAGERRLPEFEIDWLRGYQDAKPVRI 334 Query: 398 GNGAYNQRQHDIWGTMLDSVYLHAKSREQIPDALWPVLKNQVEEAIKHWKEPDRGIWEVR 457 GN A QRQ D++G ++++++L Q D W V + ++ W+EPD GIWE R Sbjct: 335 GNNAVGQRQLDVYGEVMNALHLARVGGLQADDTAWNVQRALLKHLDTIWQEPDEGIWETR 394 Query: 458 GEPQHFTSSKIMCWVALDRGSKLAELQGEKSYAQQWRAIAEEIKADVLARGVDKR-GVLT 516 G QHFT SK+M WVA DR K AE+ + +WRA +I A+V + + + Sbjct: 395 GGRQHFTFSKVMAWVAYDRAIKSAEMFELEGPLDEWRATRAQIHAEVCEKAWNPALNAFS 454 Query: 517 QRYGDDALDASLLLAVLTRFLPADDPRIRATVLAIADELTEDGLVLRYRVEETDDGLAGE 576 Q YG D LDAS+LL L FLP DPR++ TV AI +L DG V+RYR E DDGL Sbjct: 455 QCYGTDQLDASVLLMPLVAFLPPHDPRVKGTVAAIERDLMHDGFVMRYRTTEYDDGLPPG 514 Query: 577 EGTFTICSFWLVSALVEIGEISRAKHLCERLLSFASPLHLYAEEIEPRTGRHLGNFPQAF 636 EGTF CSFW+V L G + A + ERLL+ + + L AEE +P R +GNFPQAF Sbjct: 515 EGTFLACSFWMVDNLALQGRLDEAIAMYERLLALCNDVGLLAEEYDPAAKRLVGNFPQAF 574 Query: 637 THLALINAVVHVIRAEE 653 +H+AL++ +++++ E+ Sbjct: 575 SHVALVHTGLNLMKHEQ 591 Lambda K H 0.319 0.135 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1284 Number of extensions: 68 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 668 Length of database: 626 Length adjustment: 38 Effective length of query: 630 Effective length of database: 588 Effective search space: 370440 Effective search space used: 370440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory