Align alpha,alpha-trehalase (EC 3.2.1.28) (characterized)
to candidate BPHYT_RS26585 BPHYT_RS26585 trehalase
Query= BRENDA::Q3BXX2 (557 letters) >FitnessBrowser__BFirm:BPHYT_RS26585 Length = 557 Score = 590 bits (1521), Expect = e-173 Identities = 292/502 (58%), Positives = 353/502 (70%), Gaps = 6/502 (1%) Query: 41 PTPPTPDLAYPELFQAVQRGELFDDQKHFVDFLPLRDPALINADYLAQHDHPGFDLRKFV 100 P PP PD Y +LF AVQ +++ DQK FVD P DPA I Y Q +PGF L KFV Sbjct: 57 PLPPAPDKLYGDLFVAVQTAQIYPDQKTFVDATPNADPAAIVQLYEQQKHNPGFSLAKFV 116 Query: 101 DANFEE-SPPVQTDAIRQDTALREHIDLLWPKLVRSQNHVPPHSSLLALPHPYVVPGGRF 159 + F S PV T Q LREHI+ LWP L R+ PP+SSL+ LP PYVVPGGRF Sbjct: 117 NKYFTPPSEPVITPPANQ--TLREHINWLWPALTRTTTSAPPNSSLIPLPKPYVVPGGRF 174 Query: 160 REVYYWDSYFTMLGLVKSGETTLSRQMLDNFAYLIDTYGHIPNGNRTYYLSRSQPPFFSY 219 RE YYWD+YFTMLGL +SG L MLDNFAY IDT+GHIPNGNRTYYL RSQPPFFS+ Sbjct: 175 REGYYWDTYFTMLGLQESGNENLVDDMLDNFAYEIDTFGHIPNGNRTYYLDRSQPPFFSH 234 Query: 220 MVELQAGVEGEAVYQRYLPQLQKEYAYWMQGSDDLQPGQAARHVVRLADGSVLNRYWDER 279 MVEL A +EG VYQ+YLP L+KEYAYWMQG +PG A R+VV + D +VLNRYWDE Sbjct: 235 MVELAAKMEGHGVYQKYLPALRKEYAYWMQGESTTRPGSATRNVVVMPDRTVLNRYWDEL 294 Query: 280 DTPRPEAWLHDTRTAAEVKDRPAAEVYRDLRAGAESGWDYTSRWLADGQNLRTIRTTAIL 339 DTPR E++L D +TA + R +VYR+LRA AESGWD++SRW D L T+RTT+I+ Sbjct: 295 DTPRDESYLEDIQTAQQASGRNPNDVYRELRATAESGWDFSSRWFGDNMTLATVRTTSII 354 Query: 340 PIDLNSLLYHLERTLAQACAQA-GAECTRDYAALAQQRKQAIDAHLWNAAGYYADYDWQT 398 P+DLNSL++HLE T+A+ C + C ++A A +R I+ +LWN GYY DYDWQ Sbjct: 355 PVDLNSLMFHLEITIAKGCGETRDFRCVGEFAQRAAKRALGINRYLWNPNGYYGDYDWQL 414 Query: 399 RTLSNQVTAAALYPLFAGLASDDHAKRTASTVRKTLLRPGGLATTAVKTGQQWDEPNGWA 458 + TAA ++PLF G A D A +TA V+ TLL+PGGL TT T QQWD PNGWA Sbjct: 415 ARPRDNKTAAMVFPLFVGAAWPDRALKTAKQVQSTLLQPGGLVTTTYNTTQQWDAPNGWA 474 Query: 459 PLQWVAVDGLRRYGEDALARTIGERFLAQVQALFAREHKLVEKYGLE-TDAAGGGGGEYA 517 PL W A+ GL+RYG+DALA+ IG RFL+ V+ ++A + KLVEKY +E + GGGGGEY Sbjct: 475 PLHWAAIQGLKRYGQDALAQQIGTRFLSDVKGVYASDQKLVEKYVVEGSGTGGGGGGEYP 534 Query: 518 LQDGFGWTNGVTLMLLNLY-PG 538 LQDGFGWTNGVTL LL+LY PG Sbjct: 535 LQDGFGWTNGVTLKLLDLYSPG 556 Lambda K H 0.319 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 860 Number of extensions: 21 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 1 Length of query: 557 Length of database: 557 Length adjustment: 36 Effective length of query: 521 Effective length of database: 521 Effective search space: 271441 Effective search space used: 271441 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory