GapMind for catabolism of small carbon sources

 

Aligments for a candidate for acdH in Burkholderia phytofirmans PsJN

Align 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized)
to candidate BPHYT_RS02220 BPHYT_RS02220 butyryl-CoA dehydrogenase

Query= reanno::pseudo3_N2E3:AO353_25680
         (375 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS02220 BPHYT_RS02220 butyryl-CoA
           dehydrogenase
          Length = 376

 Score =  495 bits (1274), Expect = e-145
 Identities = 249/375 (66%), Positives = 298/375 (79%)

Query: 1   MLPTDEQLQISDAARQFAQERLKPFAAEWDREHRFPKEAIGEMAELGFFGMLVPEQWGGC 60
           M+   + L + DA R F +E + P AA WDRE  FPK+   ++AELG +G+LVPE +GG 
Sbjct: 1   MVLDQDHLMVRDALRTFVREAITPHAAVWDRERTFPKDVHRQLAELGAYGVLVPEAYGGA 60

Query: 61  DTGYLAYAMALEEIAAGDGACSTIMSVHNSVGCVPILKFGNDDQKERFLKPLASGAMLGA 120
               LA A+ LEEIAAGDG  ST +SV+N   C  +L +GND QK  +L PLA G MLGA
Sbjct: 61  GMDALALALILEEIAAGDGGTSTAISVNNCPVCSILLTYGNDAQKRDWLTPLARGEMLGA 120

Query: 121 FALTEPQAGSDASSLKTRARLNGDHYVLNGCKQFITSGQNAGVVIVFAVTDPSAGKRGIS 180
           F LTEPQAGSDAS+L+T A  +GD YVLNG KQFITSG+N  V IV AVTD +AGKRGIS
Sbjct: 121 FCLTEPQAGSDASALRTTATRDGDAYVLNGVKQFITSGKNGNVAIVMAVTDKAAGKRGIS 180

Query: 181 AFIVPTDSPGYKVARVEDKLGQHASDTCQILFEDVQVPVANRLGEEGEGYKIALANLEGG 240
           AFIVPTD+ GY VARVEDKLGQH+SDT QI+FED +VP AN +G EGEGY+IAL+ LEGG
Sbjct: 181 AFIVPTDTKGYVVARVEDKLGQHSSDTAQIIFEDCRVPAANLIGAEGEGYRIALSGLEGG 240

Query: 241 RVGIASQSVGMARAAFEAARDYARERESFGKPIIEHQAVAFRLADMATQIAVARQMVHYA 300
           R+GIA+QSVGMARAAFEAA  YA+ERESFG P+  HQAV FRLADMATQ+  ARQ++ +A
Sbjct: 241 RIGIAAQSVGMARAAFEAALSYAKERESFGAPLFSHQAVQFRLADMATQLEAARQLIWHA 300

Query: 301 AALRDSGKPALVEASMAKLFASEMAEKVCSTALQTLGGYGYLSDFPLERIYRDVRVCQIY 360
           A+L+D+G+P L EA+MAKLFASE AE++CS ALQ  GGYGYLSDFP+ERIYRDVRVCQIY
Sbjct: 301 ASLKDAGQPCLTEAAMAKLFASEAAERICSAALQIHGGYGYLSDFPVERIYRDVRVCQIY 360

Query: 361 EGTSDIQRMVISRNL 375
           EGTSDIQ+++I+R L
Sbjct: 361 EGTSDIQKILIARGL 375


Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 425
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 376
Length adjustment: 30
Effective length of query: 345
Effective length of database: 346
Effective search space:   119370
Effective search space used:   119370
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory