Align 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized)
to candidate BPHYT_RS17350 BPHYT_RS17350 enoyl-CoA hydratase
Query= BRENDA::A4YI89 (259 letters) >FitnessBrowser__BFirm:BPHYT_RS17350 Length = 263 Score = 150 bits (379), Expect = 3e-41 Identities = 92/265 (34%), Positives = 144/265 (54%), Gaps = 8/265 (3%) Query: 1 MEFETI--ETKKEGNLFWITLNRPDKLNALNAKLLEELDRAVSQAESDPEIRVIIITGKG 58 M +E I + ++ ITLNRPDKLN+ + +EL A+ Q E+ R +++TG G Sbjct: 1 MSYEAIRLDIDASSHVATITLNRPDKLNSFTRAMHQELSAALDQVETSGA-RALVLTGAG 59 Query: 59 KAFCAGADITQFNQLTPAEAWKFSKKGRE----IMDKIEALSKPTIAMINGYALGGGLEL 114 + FCAG D+ + TP + + ++ +++AL P IA +NG A G G L Sbjct: 60 RGFCAGQDLADLD-FTPGAMTDLGELIEQHFNPLIRRLQALPLPVIAAVNGTAAGAGANL 118 Query: 115 ALACDIRIAAEEAQLGLPEINLGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEK 174 ALACD+ +AA A + +G+ P GGT L + +G RAL + +TGD++ + AE Sbjct: 119 ALACDLVLAARSASFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLAITGDKLSAEKAES 178 Query: 175 YGLVNRVVPLANLEQETRKLAEKIAKKSPISLALIKEVVNRGLDSPLLSGLALESVGWGV 234 +GL+ + V A L + KLA ++A++ ++A IK + G L L LE Sbjct: 179 WGLIWQAVDDAELAETAAKLAAQLAQQPTRAIAAIKRAMRAGATQTLDQQLDLERDLQRE 238 Query: 235 VFSTEDKKEGVSAFLEKREPTFKGK 259 + ++ D EGV AF+EKR P F+G+ Sbjct: 239 LGASHDYAEGVQAFVEKRAPRFEGR 263 Lambda K H 0.315 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 123 Number of extensions: 7 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 259 Length of database: 263 Length adjustment: 25 Effective length of query: 234 Effective length of database: 238 Effective search space: 55692 Effective search space used: 55692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory