GapMind for catabolism of small carbon sources

 

Aligments for a candidate for PS417_12060 in Burkholderia phytofirmans PsJN

Align ABC transporter permease; SubName: Full=Monosaccharide ABC transporter membrane protein, CUT2 family; SubName: Full=Sugar ABC transporter permease (characterized, see rationale)
to candidate BPHYT_RS16055 BPHYT_RS16055 sugar ABC transporter permease

Query= uniprot:A0A1N7UKA9
         (325 letters)



>lcl|FitnessBrowser__BFirm:BPHYT_RS16055 BPHYT_RS16055 sugar ABC
           transporter permease
          Length = 335

 Score =  291 bits (745), Expect = 2e-83
 Identities = 158/325 (48%), Positives = 213/325 (65%), Gaps = 6/325 (1%)

Query: 1   MNAKTITAPV--TAAPRNRLRLSLDRFGLPLVFILLCVVMAFSSEYFMTWRNWMDILRQT 58
           +N+ T  APV  T A   R  L     GL    +++C+VM F+S+ F++  N  ++LRQ 
Sbjct: 15  VNSDTPGAPVRFTWAALKRSTLFYPFIGL----LVVCIVMVFASDSFLSGANIENVLRQV 70

Query: 59  SINGILAVGMTYVILTKGIDLSVGSILAFAGLCSAMVATQGYGLLAAVSAGMFAGAMLGV 118
           SIN I+AVGMT VILT GIDLSVGS++A AG  +A +   G   +AA++ G+  G   G 
Sbjct: 71  SINAIIAVGMTCVILTGGIDLSVGSVMALAGTLAAGLMVAGMNAVAALAIGIAVGLGFGA 130

Query: 119 VNGFMVANLSIPPFVATLGMLSIARGMTFILNDGSPITDLPDAYLALGIGKIGPIGVPII 178
            NGF VA   +PP + TL  + IARG+  I   G PI  LPD     G GKI  +  P++
Sbjct: 131 ANGFFVAFAGMPPIIVTLATMGIARGLALIYTGGYPIDGLPDWVSFFGSGKILGVQAPVV 190

Query: 179 IFAVVALIFWMVLRYTTYGRYVYAVGGNEKSARTSGIGVRKVMFSVYVVSGLLAGLAGVV 238
           I AV+ +I W++L    +GRYVYA+GGNE++ R SG+ V +V   VY ++GL +  A +V
Sbjct: 191 IMAVIYVIAWVLLERMPFGRYVYAIGGNEQATRLSGVRVARVKLIVYTIAGLTSAFAAIV 250

Query: 239 LSARTTSALPQAGVSYELDAIAAVVIGGTSLSGGTGSIVGTLFGALLIGVINNGLNLLGV 298
           L+AR  S  P AGV +ELDAIAAVV+GGTS+SGG GSI+GTL GALL+GV+NNGLN++GV
Sbjct: 251 LTARLMSGQPNAGVGFELDAIAAVVMGGTSISGGRGSIIGTLIGALLLGVLNNGLNMVGV 310

Query: 299 SSYYQQVAKGLIIVFAVLIDVWRKK 323
           + Y Q V KG II+ A+ I   R+K
Sbjct: 311 NPYVQNVIKGGIILLAIYISRDRRK 335


Lambda     K      H
   0.326    0.141    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 272
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 325
Length of database: 335
Length adjustment: 28
Effective length of query: 297
Effective length of database: 307
Effective search space:    91179
Effective search space used:    91179
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory