GapMind for catabolism of small carbon sources

 

D-alanine catabolism in Bacteroides thetaiotaomicron VPI-5482

Best path

cycA, dadA

Rules

Overview: GapMind describes D-alanine catabolism via D-alanine dehydrogenase, which forms pyruvate. This reaction is part of the MetaCyc pathway for L-alanine catabolism via D-alanine (link). In principle, D-alanine might also be catabolized via racemization to L-alanine and transamination to pyruvate, but this is not described here.

12 steps (2 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
cycA D-alanine:H+ symporter CycA
dadA D-alanine dehydrogenase
Alternative steps:
AZOBR_RS08235 D-alanine ABC transporter, permease component 1
AZOBR_RS08240 D-alanine ABC transporter, permease component 2
AZOBR_RS08245 D-alanine ABC transporter, ATPase component 1
AZOBR_RS08250 D-alanine ABC transporter, ATPase component 2 BT3837 BT3640
AZOBR_RS08260 D-alanine ABC transporter, substrate-binding component
mctP D-alanine transporter MctP
Pf6N2E2_5402 ABC transporter for D-Alanine, substrate-binding component
Pf6N2E2_5403 ABC transporter for D-Alanine, permease component 2
Pf6N2E2_5404 ABC transporter for D-Alanine, permease component 1
Pf6N2E2_5405 ABC transporter for D-Alanine, ATPase component BT2494 BT1291

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory