GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gyaR in Bacteroides thetaiotaomicron VPI-5482

Align Glyoxylate reductase; EC 1.1.1.26 (uncharacterized)
to candidate 350735 BT1207 glycerate dehydrogenase (NADH-dependent) (NCBI ptt file)

Query= curated2:A1RYE4
         (339 letters)



>lcl|FitnessBrowser__Btheta:350735 BT1207 glycerate dehydrogenase
           (NADH-dependent) (NCBI ptt file)
          Length = 318

 Score =  167 bits (424), Expect = 3e-46
 Identities = 104/275 (37%), Positives = 150/275 (54%), Gaps = 8/275 (2%)

Query: 54  LLTDK--IDAEVMDAAPNLKVISTYSVGFDHIDIPEATKRGIYVTHTPGVLTDAVAEFTV 111
           +LT+K  I+A+ M A P LK I   + G++ +D   A +RGI VT+ P   T +VA+   
Sbjct: 48  ILTNKVIINADHMTALPELKYIGVLATGYNVVDTAVAKERGIIVTNIPAYSTASVAQMVF 107

Query: 112 GLILAVTRRIVEADKIIRTGQWDKPWNPYFLTGP--ELKGKTIGLVGLGRIGVATAKRLS 169
             IL + +++    + +  G+W    +  F   P  EL+ K IGLVGLG  G  TA+   
Sbjct: 108 AHILNICQQVQHHSEEVHKGRWTNNKDFCFWDTPLIELRDKKIGLVGLGNTGYTTARVAI 167

Query: 170 SFDVKILYYDIERRWDVETVIPNMEFTDLDTLLEKSDIVSIHVPLTKETYHLINEERLRK 229
            F +++     +  + +   I  M   DLD L  + DI+S+H PLT ET+ L+N  RL  
Sbjct: 168 GFGMQVYALTSKSHFQLPPEIKKM---DLDQLFSECDIISLHCPLTPETHELVNARRLAL 224

Query: 230 MKKTAYLINTARGPVVDTEALVKALKEGWIAGAALDVFEQEPLPPNHPLTKFDNVVLAPH 289
           MK  A LINT RGP+V+ + L  AL  G I  A +DV   EP   ++PL    N  + PH
Sbjct: 225 MKPNAILINTGRGPLVNEQDLADALNSGKIYAAGVDVLSSEPPRADNPLLTAKNCYITPH 284

Query: 290 IASATIEARQRMAELAARNLIAVLKGEMPPALVNK 324
           IA A+ EAR+R+  +A  NL A + G  P  +VNK
Sbjct: 285 IAWASTEARERLMNIAISNLQAYISG-TPENVVNK 318


Lambda     K      H
   0.319    0.137    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 282
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 339
Length of database: 318
Length adjustment: 28
Effective length of query: 311
Effective length of database: 290
Effective search space:    90190
Effective search space used:    90190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory