Alignments for a candidate for dauA in Bacteroides thetaiotaomicron VPI-5482

GapMind for catabolism of small carbon sources

Alignments for a candidate for dauA in Bacteroides thetaiotaomicron VPI-5482

Align C4-dicarboxylic acid transporter DauA; Dicarboxylic acid uptake system A (characterized)
to candidate 352708 BT3181 putative sulfate transporter, permease (NCBI ptt file)

Query= SwissProt::P0AFR2
         (559 letters)



>FitnessBrowser__Btheta:352708
          Length = 559

 Score =  355 bits (910), Expect = e-102
 Identities = 201/567 (35%), Positives = 323/567 (56%), Gaps = 43/567 (7%)

Query: 12  FRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIV 71
           F+  + +C K  Y+   F  DL+AG+ VGI+A+PLA+A  I SGV+P+ G+ TA +AG +
Sbjct: 6   FKPKLVSCLKN-YSKETFMADLMAGVIVGIVALPLAIAFGIASGVSPEKGIITAIIAGFI 64

Query: 72  IALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYI 131
           I+L GGS+  + GPT AF+VI+Y + QQ+G AGL+VATL++G+ LIL+G+ + G +I++I
Sbjct: 65  ISLLGGSKVQIGGPTGAFIVIIYGIIQQYGEAGLIVATLMAGVLLILLGVFKLGAVIKFI 124

Query: 132 PVSVTLGFTSGIGITIGTMQIKDFLGLQMA--HVPEHYLQKVGALFMALPTINVGDAAIG 189
           P  + +GFTSGI +TI T QI D  GL      VP  ++ K    F    T+N  +  + 
Sbjct: 125 PYPIIVGFTSGIAVTIFTTQIADIFGLSFGGEKVPGDFVGKWMIYFRHFDTVNWWNTIVS 184

Query: 190 IVTLGILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGG--HVATIGSQFHYVLADGSQG 247
           IV++ I+   PR   ++PG L A++     + ++   GG   + TIG +F          
Sbjct: 185 IVSIIIIAITPRFSKKIPGSLIAIIVVTVAVYLMKTYGGIDCIPTIGDRF---------- 234

Query: 248 NGIPQLLPQLVLPWDLPNSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTK 307
             I   LP  V+P         L W++I+ L P A ++A+LGAIESLL A V DG+ G +
Sbjct: 235 -TIKSELPDAVVP--------ALDWEAIKNLFPVAITIAVLGAIESLLSATVADGVIGDR 285

Query: 308 HKANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVL 367
           H +N+EL+ QG  NI+AP FGGI AT AIAR+  N+  G  +PI+ +IH+I+++L LL L
Sbjct: 286 HDSNTELIAQGAANIVAPLFGGIPATGAIARTMTNINNGGKTPIAGIIHAIVLLLILLFL 345

Query: 368 APLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAI 427
            PL  ++P++ +A +L++V++NMS   +V   L   PK D+ V+L+   LTV+FD+ +AI
Sbjct: 346 MPLAQYIPMACLAGVLVIVSYNMS-GWRVFKALLKNPKSDVTVLLITFFLTVIFDLTVAI 404

Query: 428 SVGIVLASLLFMRRIARMTRLAPVV-----------------VDVPDDVLVLRVIGPLFF 470
            VG+++A +LFM+R+   T ++ +                  + +P  V V  + GP FF
Sbjct: 405 EVGLIIACVLFMKRVMETTEISVITDEIDPNKESDIAVNEENIMIPKGVEVYEITGPYFF 464

Query: 471 AAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAFQRFVK-RLPEGCELRVCNVEFQ 529
             A      +    +   + I++   VP +D+ G+       +    E   + +  V  +
Sbjct: 465 GIATKFEETMAQLGDRPNVRIIRMRKVPFIDSTGIHNLTTLCEMSQKEKITVILSGVNEK 524

Query: 530 PLRTMARAGIQPIPGRLAFFPNRRAAM 556
             + + ++G   + G+    PN + A+
Sbjct: 525 VYKVLEKSGFYELLGKENICPNFKIAL 551


Lambda     K      H
   0.328    0.142    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 638
Number of extensions: 26
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 559
Length adjustment: 36
Effective length of query: 523
Effective length of database: 523
Effective search space:   273529
Effective search space used:   273529
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources