GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fucU in Bacteroides thetaiotaomicron VPI-5482

Align L-fucose mutarotase (EC 5.1.3.29) (characterized)
to candidate 353703 BT4177 conserved hypothetical protein (NCBI ptt file)

Query= reanno::Smeli:SM_b21108
         (109 letters)



>FitnessBrowser__Btheta:353703
          Length = 104

 Score = 57.8 bits (138), Expect = 4e-14
 Identities = 28/80 (35%), Positives = 47/80 (58%), Gaps = 3/80 (3%)

Query: 1  MQRMGMVIGLEPSKIAEYKRLHAAVWPEILALISECNITNYSIFLKEPENLLFGYWEYVG 60
          M+R    + L+P   AEY++ HAA+WPE+ AL+S+  +++YSI+  +  N+LF + +  G
Sbjct: 1  MKREAFKMYLKPGYEAEYEKRHAAIWPELKALLSKNGVSDYSIYWDKETNILFAFQKTEG 60

Query: 61 EDFEADMTKMAAHPKNQEWW 80
          E    D+         Q+WW
Sbjct: 61 EGGSQDLGNTEI---VQKWW 77


Lambda     K      H
   0.321    0.134    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 43
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 109
Length of database: 104
Length adjustment: 12
Effective length of query: 97
Effective length of database: 92
Effective search space:     8924
Effective search space used:     8924
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.4 bits)
S2: 40 (20.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory