Alignments for a candidate for uxaB in Bacteroides thetaiotaomicron VPI-5482

GapMind for catabolism of small carbon sources

Alignments for a candidate for uxaB in Bacteroides thetaiotaomicron VPI-5482

Align tagaturonate reductase (EC 1.1.1.58) (characterized)
to candidate 350353 BT0825 altronate oxidoreductase (NCBI ptt file)

Query= ecocyc::ALTRO-OXIDOREDUCT-MONOMER
         (483 letters)



>FitnessBrowser__Btheta:350353
          Length = 479

 Score =  403 bits (1035), Expect = e-117
 Identities = 217/482 (45%), Positives = 298/482 (61%), Gaps = 8/482 (1%)

Query: 1   MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDLNSGVVVVRPIETSFPP 60
           MK LN+   P  Q PERIIQFGEGNFLRAFVDW I  +N+ TD NS VVVV+PI+     
Sbjct: 1   MKALNKETAPKVQRPERIIQFGEGNFLRAFVDWIIYNMNQKTDFNSSVVVVQPIDKGMVD 60

Query: 61  SLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEYDEFLKLAHNPEMRFVFSN 120
            L+ QD LY   ++GL +KGE V+   +I  ++R ++ Y++ DEF+KLA  PEMRFV SN
Sbjct: 61  MLNAQDDLYHVNLQGL-DKGEVVNSLTMIDVISRALNPYTQNDEFMKLAEQPEMRFVISN 119

Query: 121 TTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGALDKGWIIIPCELIDYNGDAL 180
           TTEAGI++    K +DAPA SYP KLT+LL+ RF  FNG   KG II PCELI  NG  L
Sbjct: 120 TTEAGIAFDPTCKLEDAPASSYPGKLTQLLYHRFKTFNGDKTKGLIIFPCELIFLNGHKL 179

Query: 181 RELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTGYPRDEVAKLEEELGYHDGFLDT 240
           +E + +Y   W L   F  W ++A    +TLVDRIV G+PR ++A ++E++ Y D  +  
Sbjct: 180 KETIYQYIDLWNLGNEFKTWFEEACGVYATLVDRIVPGFPRKDIAAIKEKIQYDDNLVVQ 239

Query: 241 AEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVAILNGAHTALVPVAFQAG 300
           AE F+L+VI+ P+ +A E   DK  LNVL V    PY ERKV +LNG HT L PVA+ +G
Sbjct: 240 AEIFHLWVIEAPQEVAKEFPADKAGLNVLFVPSEAPYHERKVTLLNGPHTVLSPVAYLSG 299

Query: 301 LDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRDELESFASAVTGRFRNPYIKHQLLSIA 360
           ++ V +A     I  ++ K +++E++  L+LP++EL+ FA  V  RF NP++ H + SI 
Sbjct: 300 VNIVRDACQHEVIGKYIHKVMFDELMETLNLPKEELKKFAEDVLERFNNPFVDHAVTSIM 359

Query: 361 LNGMTKFRTRILPQLLAGQKANGTLPARLTFALAALIAFYRG--ERNGETYPVQDDAHWL 418
           LN   K+ TR LP L    +  G LP  L   LAA+I +Y+G    +G      D    +
Sbjct: 360 LNSFPKYETRDLPGLKTYLERKGELPKGLVLGLAAIITYYKGGVRADGAEIVPNDAPEIM 419

Query: 419 ERYQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREA 478
              ++LW+        T+++   VLA +  W +DL ++PGL   V  DLD+I EKGM E 
Sbjct: 420 NLLKELWATG-----CTKKVTEGVLAAEFIWGEDLNKIPGLAAAVKADLDSIQEKGMLET 474

Query: 479 VR 480
           V+
Sbjct: 475 VK 476


Lambda     K      H
   0.321    0.138    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 651
Number of extensions: 34
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 483
Length of database: 479
Length adjustment: 34
Effective length of query: 449
Effective length of database: 445
Effective search space:   199805
Effective search space used:   199805
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources