Alignments for a candidate for lacL in Bacteroides thetaiotaomicron VPI-5482

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacL in Bacteroides thetaiotaomicron VPI-5482

Align β-galactosidase (LacLM;LacL+LacM) (EC 3.2.1.23) (characterized)
to candidate 352820 BT3293 beta-galactosidase (NCBI ptt file)

Query= CAZy::AEJ32720.1
         (626 letters)



>FitnessBrowser__Btheta:352820
          Length = 1421

 Score =  388 bits (997), Expect = e-112
 Identities = 219/615 (35%), Positives = 329/615 (53%), Gaps = 45/615 (7%)

Query: 28  YRDQEEA---ALEKSSYVQNLNGRWGFKFSKNPMERPVDFYKLDFDRNDFGEIEVPSEIE 84
           + D+++A    +E+S Y + LNG W F +  +P +RP DF K ++D + +  I+VP+  +
Sbjct: 50  FTDEQQAMNLTIEESPYYKTLNGIWKFHWVADPKDRPQDFCKPEYDVSQWDNIKVPATWQ 109

Query: 85  LS------NFAQINYTNITMP----------WTGKIYRRPA-YTLGDNKEEGSFSQGKDN 127
           +       N+ +  Y N+  P          W   I  RP+ YT               N
Sbjct: 110 IEAVRHNKNWDKPLYCNVIYPFCEWDWKKIQWPNVIQPRPSNYTFAT----------MPN 159

Query: 128 TVGSYVRHFTLAEGLKNHDVHVVFEGVERAMYVWLNGHFIGYAEDSFTPSEFDLTPYLVD 187
            VGSY R F L +  K  D+ + F GVE   Y+W+NG  +GY+EDS+ P+EF+LTPYL  
Sbjct: 160 PVGSYRREFILPDSWKGRDIFIRFNGVEAGFYIWVNGKKVGYSEDSYLPAEFNLTPYLKA 219

Query: 188 GDNLLAVEVYKHATSSWIEDQDMFRFSGIFRDVNLVAQPSIHVQDLKIDARVADDMKTGS 247
           G N+LAVEVY+    S++E QD +RFSGIFRDV L + P   ++D      +  + K  S
Sbjct: 220 GKNVLAVEVYRFTDGSFLECQDFWRFSGIFRDVFLWSAPKTQIRDFFFRTDLDKEYKNAS 279

Query: 248 LGLVLKVVGQPGS--VQVEVADQTGAAVLNRQLNADGNWTMAPVQLVGIHLWDNHHPYLY 305
           + L + + G+  +  +QV+V DQ G  +  +   A         ++V    W    P LY
Sbjct: 280 VSLDIDITGKRSNNEIQVKVTDQNGKEIATQNARAVTGTNKLQFEVVNPLKWTAETPNLY 339

Query: 306 QLTLTVRDATGRVVEVIPYQFGFRRVEIDQDKVLRLNGKRLIINGVNRHEWNCHRGRAVT 365
            LT+ ++   G+ V++   + GFR++E+ QD  L +NGK  +  GV+RH+ +   GR V+
Sbjct: 340 NLTILLKQ-KGKTVDIRSVKVGFRKIELAQDGRLLINGKSTLFKGVDRHDHSSENGRTVS 398

Query: 366 IEDMHTDLGIFKENNINAVRTSHYPDQIPWYYLCDREGIYMMAENNLESHATWQKFGQDE 425
            E+M  D+ + K  NINAVRTSHYP+   +Y LCDR GIY+++E N+E H          
Sbjct: 399 KEEMEKDVQLMKSLNINAVRTSHYPNNPYFYDLCDRYGIYVLSEANVECHGLM------- 451

Query: 426 PSYNVPGSLPQWKEAVVDRARSNYETFKNHTAILFWSVGNESYAGEDILAMNNYYKEVDD 485
                  S P W +A  +R+ +    +KNH +I+ WS+GNES  G +  +     K++DD
Sbjct: 452 ----ALSSEPSWVKAFTERSENMVRRYKNHASIVMWSLGNESGNGINFKSAAEAVKKLDD 507

Query: 486 TRPVHYEGVVHTKEYRDQISDFESWMYLPPKE-VEAYLKKNPDKPFIECEYMHSMGNSVG 544
           TRP HYEG     +    +     W+    KE ++ +      KP + CEY H+MGNS+G
Sbjct: 508 TRPTHYEGNSSYCDVTSSMYPDVQWLESVGKERLQKFQNGETVKPHVVCEYAHAMGNSIG 567

Query: 545 GMDSYIKLLDKYPQYCGGFIWDFVDQAIEVVDPVTGQKSMRYGGDFDDHHADNEFSGDGI 604
               Y +  ++YP   GGFIWD+VDQ+I++  P      M +GGDF D   D  F  +G+
Sbjct: 568 NFKEYWETYERYPALVGGFIWDWVDQSIKMPAPDGSGYYMAFGGDFGDTPNDGNFCTNGV 627

Query: 605 CFADRTPKPAMQEVK 619
            F+DRT      EVK
Sbjct: 628 IFSDRTYSAKAYEVK 642


Lambda     K      H
   0.319    0.137    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2477
Number of extensions: 131
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 626
Length of database: 1421
Length adjustment: 44
Effective length of query: 582
Effective length of database: 1377
Effective search space:   801414
Effective search space used:   801414
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources