GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natE in Bacteroides thetaiotaomicron VPI-5482

Align NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate 353363 BT3837 ABC transporter ATP-binding protein (NCBI ptt file)

Query= TCDB::Q8YT15
         (247 letters)



>FitnessBrowser__Btheta:353363
          Length = 255

 Score =  123 bits (308), Expect = 4e-33
 Identities = 69/217 (31%), Positives = 117/217 (53%), Gaps = 2/217 (0%)

Query: 27  ILQGVNFRVESGELVTVIGPNGAGKSTLAKTIFGLLTPHTGKITFKGKNIAGLKSNQIVR 86
           ++  V+  V+ GE+V ++GPNGAGK+T      GL+TP+ G+I      I      +  +
Sbjct: 22  VVSHVSIDVKQGEIVGLLGPNGAGKTTSFYMTVGLITPNEGRIFLDDLEITKFPVYKRAQ 81

Query: 87  LGMCYVPQIANVFPSLSVEENLEMGAFIRNDSLQPLKDKIFAMFP--RLSDRRRQRAGTL 144
            G+ Y+ Q A+VF  +SVE+N+     + N      KDK+ ++    RL   R+ +   L
Sbjct: 82  TGIGYLAQEASVFRQMSVEDNIASVLEMTNKPKDYQKDKLESLIAEFRLQKVRKNKGNQL 141

Query: 145 SGGERQMLAMGKALMLEPSLLVLDEPSAALSPILVTQVFEQVKQINQEGTAIILVEQNAR 204
           SGGER+   + + L ++P  ++LDEP A + PI V  + + V ++  +   I++ + N +
Sbjct: 142 SGGERRRTEIARCLAIDPKFIMLDEPFAGVDPIAVEDIQQIVWKLKDKNIGILITDHNVQ 201

Query: 205 KALEMADRGYVLESGRDAISGPGQELLTDPKVAELYL 241
           + L + DR Y+L  G+    G  +EL  +  V E YL
Sbjct: 202 ETLSITDRAYLLFEGKILFQGTPEELAENKIVREKYL 238


Lambda     K      H
   0.317    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 156
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 247
Length of database: 255
Length adjustment: 24
Effective length of query: 223
Effective length of database: 231
Effective search space:    51513
Effective search space used:    51513
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory