Align α-glucosidase (BT3299;BT_3299) (EC 3.2.1.20) (characterized)
to candidate 352826 BT3299 alpha-glucosidase II (NCBI ptt file)
Query= CAZy::AAO78405.1 (683 letters) >FitnessBrowser__Btheta:352826 Length = 683 Score = 1419 bits (3673), Expect = 0.0 Identities = 683/683 (100%), Positives = 683/683 (100%) Query: 1 MVGDGIAKFIPEGFDAQKIPSFAIEKEPREQGALPADWVLVPEFSLTDGKANASLTVPEG 60 MVGDGIAKFIPEGFDAQKIPSFAIEKEPREQGALPADWVLVPEFSLTDGKANASLTVPEG Sbjct: 1 MVGDGIAKFIPEGFDAQKIPSFAIEKEPREQGALPADWVLVPEFSLTDGKANASLTVPEG 60 Query: 61 TSIYGGGEVTGSLLRNGKTIKLWNTDSGAYGVDKGTRLYQSHPWMMGVRKDGTAFGILFD 120 TSIYGGGEVTGSLLRNGKTIKLWNTDSGAYGVDKGTRLYQSHPWMMGVRKDGTAFGILFD Sbjct: 61 TSIYGGGEVTGSLLRNGKTIKLWNTDSGAYGVDKGTRLYQSHPWMMGVRKDGTAFGILFD 120 Query: 121 TTWKAELSSTDEKIELKSEGIPFRVFIIDRESPQAVIRGLSELTGTMPMIPRWALGYQQC 180 TTWKAELSSTDEKIELKSEGIPFRVFIIDRESPQAVIRGLSELTGTMPMIPRWALGYQQC Sbjct: 121 TTWKAELSSTDEKIELKSEGIPFRVFIIDRESPQAVIRGLSELTGTMPMIPRWALGYQQC 180 Query: 181 RFSYSPDSRVIEIADTFRLKRIPCDVIWMDIDYMDGYRIFTFNPKSFPNPKAVNRDLHIR 240 RFSYSPDSRVIEIADTFRLKRIPCDVIWMDIDYMDGYRIFTFNPKSFPNPKAVNRDLHIR Sbjct: 181 RFSYSPDSRVIEIADTFRLKRIPCDVIWMDIDYMDGYRIFTFNPKSFPNPKAVNRDLHIR 240 Query: 241 GFHSAWMIDPGAKVDPNYFVYKSGTENDVWVKTADGKNFHGDAWPGAAAFPDFTSPKVNK 300 GFHSAWMIDPGAKVDPNYFVYKSGTENDVWVKTADGKNFHGDAWPGAAAFPDFTSPKVNK Sbjct: 241 GFHSAWMIDPGAKVDPNYFVYKSGTENDVWVKTADGKNFHGDAWPGAAAFPDFTSPKVNK 300 Query: 301 WWRNLYKDFLAQGVDGVWNDVNEPQINDTPNKTMPEDNLHRGGGKLPAGTHLQYHNVYGF 360 WWRNLYKDFLAQGVDGVWNDVNEPQINDTPNKTMPEDNLHRGGGKLPAGTHLQYHNVYGF Sbjct: 301 WWRNLYKDFLAQGVDGVWNDVNEPQINDTPNKTMPEDNLHRGGGKLPAGTHLQYHNVYGF 360 Query: 361 LMVKASREGILDARPEKRPFILTRSNFLGGQRYAATWTGDNGSCWDHLKMSVPMSLTLGL 420 LMVKASREGILDARPEKRPFILTRSNFLGGQRYAATWTGDNGSCWDHLKMSVPMSLTLGL Sbjct: 361 LMVKASREGILDARPEKRPFILTRSNFLGGQRYAATWTGDNGSCWDHLKMSVPMSLTLGL 420 Query: 421 SGQPFSGADIGGFLFNADADLFGNWIGFGAFYPFARGHACAGTNNKEPWVFGQKVEDASR 480 SGQPFSGADIGGFLFNADADLFGNWIGFGAFYPFARGHACAGTNNKEPWVFGQKVEDASR Sbjct: 421 SGQPFSGADIGGFLFNADADLFGNWIGFGAFYPFARGHACAGTNNKEPWVFGQKVEDASR 480 Query: 481 IALERRYILLPYFYTLLHEASTNGMPIMRPVFFSDPKDLSLRAEEEAFLVGDNLLIIPAF 540 IALERRYILLPYFYTLLHEASTNGMPIMRPVFFSDPKDLSLRAEEEAFLVGDNLLIIPAF Sbjct: 481 IALERRYILLPYFYTLLHEASTNGMPIMRPVFFSDPKDLSLRAEEEAFLVGDNLLIIPAF 540 Query: 541 ANQPALPKGIWKELSLVEGDQNDKYQAKMKIRGGAIIPTGKIIQNTTENSLDPLTLLVCL 600 ANQPALPKGIWKELSLVEGDQNDKYQAKMKIRGGAIIPTGKIIQNTTENSLDPLTLLVCL Sbjct: 541 ANQPALPKGIWKELSLVEGDQNDKYQAKMKIRGGAIIPTGKIIQNTTENSLDPLTLLVCL 600 Query: 601 DEQGKASGNMYWDAGDGWSYKKGDYSLLQFVAERNGDKVTVKLTKKTGKYNTENKDMAVI 660 DEQGKASGNMYWDAGDGWSYKKGDYSLLQFVAERNGDKVTVKLTKKTGKYNTENKDMAVI Sbjct: 601 DEQGKASGNMYWDAGDGWSYKKGDYSLLQFVAERNGDKVTVKLTKKTGKYNTENKDMAVI 660 Query: 661 KIITDQGIRQASGNLVEGIEIRL 683 KIITDQGIRQASGNLVEGIEIRL Sbjct: 661 KIITDQGIRQASGNLVEGIEIRL 683 Lambda K H 0.319 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2026 Number of extensions: 99 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 683 Length of database: 683 Length adjustment: 39 Effective length of query: 644 Effective length of database: 644 Effective search space: 414736 Effective search space used: 414736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory