GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mtlK in Bacteroides thetaiotaomicron VPI-5482

Align ABC transporter for D-mannitol and D-mannose, ATPase component (characterized)
to candidate 351279 BT1751 Glycine betaine transport ATP-binding protein (NCBI ptt file)

Query= reanno::pseudo3_N2E3:AO353_25895
         (367 letters)



>FitnessBrowser__Btheta:351279
          Length = 408

 Score =  171 bits (433), Expect = 3e-47
 Identities = 89/222 (40%), Positives = 138/222 (62%), Gaps = 6/222 (2%)

Query: 19  IKGIDLEVNDREFVVFVGPSGCGKSTLLRLIAGLEEVTAGTIELDGRDITEVSPA----- 73
           +K  +L +N+ E  V +G SG GKSTLLR I  L   T+G + ++G DI +VS       
Sbjct: 43  VKDANLSINEGEIFVIMGLSGSGKSTLLRCINRLIRPTSGEVIINGTDIAKVSDKELLQI 102

Query: 74  -KRDLAMVFQTYALYPHMSVRKNMSFALDLAGVNKAEVEKKVNEAARILELGPMLERKPK 132
            +++LAMVFQ + L PH SV  N++F L+L GV K E E+K  E+ +++ L     +   
Sbjct: 103 RRKELAMVFQNFGLLPHRSVLHNIAFGLELQGVKKGEREQKAMESMQLVGLKGYENQMVS 162

Query: 133 QLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQMRLELARLHKELQATMIYVTH 192
           +LSGG +QRV + RA+  NP++ L DE  S LD  +RVQM+ EL  L  +++ T++++TH
Sbjct: 163 ELSGGMQQRVGLARALANNPEVLLMDEAFSALDPLIRVQMQDELLTLQSKMKKTIVFITH 222

Query: 193 DQVEAMTLADKVVVLNGGRIEQVGSPLELYHQPANLFVAGFL 234
           D  EA+ L D++ ++  G I Q+G+  E+  +PAN +V  F+
Sbjct: 223 DLSEAIKLGDRIAIMKDGEIVQIGTSEEILTEPANAYVERFV 264


Lambda     K      H
   0.321    0.137    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 290
Number of extensions: 17
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 367
Length of database: 408
Length adjustment: 30
Effective length of query: 337
Effective length of database: 378
Effective search space:   127386
Effective search space used:   127386
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory