GapMind for catabolism of small carbon sources

 

Aligments for a candidate for HSERO_RS00900 in Bacteroides thetaiotaomicron VPI-5482

Align ABC-type branched-chain amino acid transport system, ATPase component protein (characterized, see rationale)
to candidate 350090 BT0562 putative ABC transporter ATP-binding protein (NCBI ptt file)

Query= uniprot:D8IUY7
         (241 letters)



>lcl|FitnessBrowser__Btheta:350090 BT0562 putative ABC transporter
           ATP-binding protein (NCBI ptt file)
          Length = 489

 Score = 98.2 bits (243), Expect = 3e-25
 Identities = 58/191 (30%), Positives = 110/191 (57%), Gaps = 6/191 (3%)

Query: 5   ILKVQQLSVAYGGIQAVKGIDLEVNEGELVTLIGANGAGKTTTLKAITGTLPASRVEGHI 64
           I+ V+++S +YG ++A+K +   V +GE+  LIG +GAGK+T  + +T  L A +    +
Sbjct: 7   IVVVKEISKSYGKVEALKEVSFAVEQGEVFGLIGPDGAGKSTLFRILTTLLLADKGTATV 66

Query: 65  EYLGQPLKGKKSFELVKDKLAMVPEGRGVFTRMSIQENLLMGAYTSDDKGQIAADIDKWF 124
             L      K+    ++ K+  +P    ++  +S++ENL   A       Q   D+ K  
Sbjct: 67  NGLDVVTDYKQ----IRTKVGYMPGRFSLYQDLSVEENLEFFATVFHTLIQENYDLIK-- 120

Query: 125 AVFPRLKERAAQMAGTLSGGEQQMLAMARALMSHPKLLLLDEPSMGLSPIMVEKIFEVIR 184
            ++ +++    + AG LSGG +Q LA++ +L+  P +L LDEP+ G+ P+  ++ ++++R
Sbjct: 121 DIYQQIEPFKKRRAGALSGGMKQKLALSCSLIHKPDILFLDEPTTGVDPVSRKEFWQMLR 180

Query: 185 NVSAQGITILL 195
           N+  QGITI++
Sbjct: 181 NLRKQGITIIV 191



 Score = 74.3 bits (181), Expect = 4e-18
 Identities = 60/224 (26%), Positives = 105/224 (46%), Gaps = 17/224 (7%)

Query: 1   MTTNILKVQQLSVAYGGIQAVKGIDLEVNEGELVTLIGANGAGKTTTLKAITG----TLP 56
           M   +++V+QL+ ++G   AV  I  +V  GE+   +GANGAGKTT ++ + G    T  
Sbjct: 243 MAAPVIEVEQLTKSFGHFTAVDHISFQVQRGEIFGFLGANGAGKTTAMRMLCGLSRPTSG 302

Query: 57  ASRVEGHIEYLGQPLKGKKSFELVKDKLAMVPEGRGVFTRMSIQEN--LLMGAYTSDDKG 114
             +V G+  +        +  E VK  +  + +   ++  + + EN  L  G Y   +  
Sbjct: 303 VGKVAGYDIF--------REAEQVKRHIGYMSQKFSLYEDLKVWENIRLFAGIYGMKEM- 353

Query: 115 QIAADIDKWFAVFPRLKERAAQMAGTLSGGEQQMLAMARALMSHPKLLLLDEPSMGLSPI 174
           +I    D+         ER   +   L  G +Q LA + ++   PK++ LDEP+ G+ P 
Sbjct: 354 EIEEKTDELLERLGFADERDT-LVKNLPLGWKQKLAFSVSIFHEPKIVFLDEPTGGVDPA 412

Query: 175 MVEKIFEVIRNVSAQGITILLVEQNAKLALEAAHRGYVMESGLI 218
              + +E+I   + +GIT+ +       A E  +R  +M  G I
Sbjct: 413 TRRQFWELIYQAADRGITVFVTTHYMDEA-EYCNRISIMVDGQI 455


Lambda     K      H
   0.317    0.134    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 235
Number of extensions: 7
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 241
Length of database: 489
Length adjustment: 29
Effective length of query: 212
Effective length of database: 460
Effective search space:    97520
Effective search space used:    97520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory