Align β-fructofuranosidase (BT1759) (EC 3.2.1.26|3.2.1.64|3.2.1.80) (characterized)
to candidate 351287 BT1759 levanase precursor (2,6-beta-D-fructofuranosidase) (NCBI ptt file)
Query= CAZy::AAO76866.1 (610 letters) >FitnessBrowser__Btheta:351287 Length = 610 Score = 1266 bits (3277), Expect = 0.0 Identities = 610/610 (100%), Positives = 610/610 (100%) Query: 1 MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED 60 MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED Sbjct: 1 MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED 60 Query: 61 ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL 120 ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL Sbjct: 61 ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL 120 Query: 121 SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY 180 SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY Sbjct: 121 SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY 180 Query: 181 GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT 240 GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT Sbjct: 181 GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT 240 Query: 241 QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM 300 QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM Sbjct: 241 QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM 300 Query: 301 QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS 360 QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS Sbjct: 301 QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS 360 Query: 361 ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND 420 ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND Sbjct: 361 ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND 420 Query: 421 VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP 480 VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP Sbjct: 421 VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP 480 Query: 481 NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP 540 NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP Sbjct: 481 NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP 540 Query: 541 MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV 600 MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV Sbjct: 541 MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV 600 Query: 601 DSFVVYKLKK 610 DSFVVYKLKK Sbjct: 601 DSFVVYKLKK 610 Lambda K H 0.318 0.135 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1576 Number of extensions: 48 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 610 Length of database: 610 Length adjustment: 37 Effective length of query: 573 Effective length of database: 573 Effective search space: 328329 Effective search space used: 328329 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory