GapMind for catabolism of small carbon sources

 

Finding step gtsD for sucrose catabolism in Bacteroides thetaiotaomicron VPI-5482

4 candidates for gtsD: glucose ABC transporter, ATPase component (GtsD)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med BT1291 spermidine/putrescine transport ATP-binding protein (NCBI ptt file) Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 40% 95% 243 Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD 53% 366.3
lo BT1751 Glycine betaine transport ATP-binding protein (NCBI ptt file) GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) 35% 72% 177.2 Glycine betaine/carnitine transport ATP-binding protein GbuA; EC 7.6.2.9 53% 417.5
lo BT0694 ABC transporter, ATP-binding protein (NCBI ptt file) Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 36% 56% 135.2 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 48% 220.7
lo BT1535 ABC transporter ATP-binding protein (NCBI ptt file) ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 36% 51% 125.9 Uncharacterized ABC transporter ATP-binding protein YknY; EC 7.6.2.- 51% 215.3

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step gtsD

Or cluster all characterized gtsD proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory