Alignments for a candidate for TRET1 in Bacteroides thetaiotaomicron VPI-5482

GapMind for catabolism of small carbon sources

Alignments for a candidate for TRET1 in Bacteroides thetaiotaomicron VPI-5482

Align Facilitated trehalose transporter Tret1; BmTRET1 (characterized)
to candidate 350322 BT0794 D-xylose-proton symporter (D-xylose transporter) (NCBI ptt file)

Query= SwissProt::A9ZSY3
         (505 letters)



>FitnessBrowser__Btheta:350322
          Length = 484

 Score =  168 bits (426), Expect = 3e-46
 Identities = 129/435 (29%), Positives = 216/435 (49%), Gaps = 63/435 (14%)

Query: 95  GLMPLAALVGGIVGGPL----IEYLGRKKTI-----------MGTAVPFTIGWMLIANAI 139
           G+   +AL+G ++GG L       LGR+ ++           +G+  P  + +      +
Sbjct: 59  GITSSSALIGCVLGGALSGVFASRLGRRNSLRLAAVLFFLSALGSYYPEVLFFEYGKPNM 118

Query: 140 NVVMVF-AGRVICGVCVGIVSLAFPVYIGETIQPEVRGAL----------GLLPTAFGN- 187
           ++++ F   RV+ G+ VG+ S   P+YI E     +RG L          G+L   F N 
Sbjct: 119 DLLIAFNLYRVLGGIGVGLASAVCPMYIAEIAPSNIRGTLVSCNQFAIIFGMLVVYFVNY 178

Query: 188 -------TGILLAFLVG-----------SYLDWSNLAFFGAAIPVPFF-LLMILTPETPR 228
                    I+L    G           +  +     F   A P  FF LL+   P+TPR
Sbjct: 179 LIMGDHQNPIILKDAAGVLSVSAESDMWTVQEGWRYMFGSEAFPAAFFGLLLFFVPKTPR 238

Query: 229 WYVSKARVQEARKSLRWLRGKNVNIEKEMRDLTISQTESDRTGGNAFKQLFSKRYLPAVM 288
           + V   + ++A   L  + GK    ++ + D  I  T  ++T     ++LF+   +  ++
Sbjct: 239 YLVLVQQEEKAYTILEKINGKK-KAQEILND--IKATAQEKT-----EKLFTYG-VTVIV 289

Query: 289 ISLGLMLFQQLTGINAVIFYAASIFQMSGSSVDENLASIIIGVVNFISTFIATMLIDRLG 348
           I + L +FQQ  GINAV++YA  IF+ +G+     + ++I+G+VN I T +A   +DR G
Sbjct: 290 IGILLSVFQQAIGINAVLYYAPRIFENAGAEGGGMMQTVIMGIVNIIFTLVAIFTVDRFG 349

Query: 349 RKVLLYISSVAMITTLLALGAYFYLKQNHIDVTAYGWLPLACLVIYVLGFSIGFGPIPWL 408
           RK LL I S+ M     A+GA+     + + +   G LP+  +++Y   F + +GPI W+
Sbjct: 350 RKPLLIIGSIGM-----AVGAFAVAMCDSMAIK--GVLPVLSIIVYAAFFMMSWGPICWV 402

Query: 409 MLGEILPSKIRGTAASLATGFNWTCTFIVTKTFQNIIDAIYMHGTLWLFAVICIGGLLFV 468
           ++ EI P+ IRG A ++A  F W   +IV+ TF  + D   M     L+ +IC+   +FV
Sbjct: 403 LISEIFPNTIRGKAVAIAVAFQWIFNYIVSSTFPALYDFSPMF-AYSLYGIICVAAAIFV 461

Query: 469 IFFVPETKGKSLEEI 483
             +VPETKGK+LE++
Sbjct: 462 WRWVPETKGKTLEDM 476



 Score = 33.5 bits (75), Expect = 2e-05
 Identities = 45/160 (28%), Positives = 71/160 (44%), Gaps = 23/160 (14%)

Query: 280 SKRYLPAV--MISLGLMLFQQLTGINA-------VIFYAASIFQMSGSSVDENLASIIIG 330
           SK YL ++  +  LG +LF   T + +         F +AS FQ +        +S +IG
Sbjct: 9   SKLYLYSITSVAILGGLLFGYDTAVISGAEKGLEAFFLSASDFQYNKVMHGITSSSALIG 68

Query: 331 VVNFISTFIATMLIDRLGRKVLLYISSVAMITTLLALGAYFYLKQNHIDVTAYGWLPLAC 390
            V  +   ++ +   RLGR+  L +++V     L ALG+Y+      +    YG   +  
Sbjct: 69  CV--LGGALSGVFASRLGRRNSLRLAAVLFF--LSALGSYY----PEVLFFEYGKPNMDL 120

Query: 391 LVIYVL-----GFSIGFGPIPWLM-LGEILPSKIRGTAAS 424
           L+ + L     G  +G       M + EI PS IRGT  S
Sbjct: 121 LIAFNLYRVLGGIGVGLASAVCPMYIAEIAPSNIRGTLVS 160


Lambda     K      H
   0.325    0.140    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 597
Number of extensions: 28
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 505
Length of database: 484
Length adjustment: 34
Effective length of query: 471
Effective length of database: 450
Effective search space:   211950
Effective search space used:   211950
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources