GapMind for catabolism of small carbon sources

 

Protein H281DRAFT_03528 in Paraburkholderia bryophila 376MFSha3.1

Annotation: FitnessBrowser__Burk376:H281DRAFT_03528

Length: 282 amino acids

Source: Burk376 in FitnessBrowser

Candidate for 6 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-fucose catabolism KDF-hydrolase hi 2,4-diketo-3-deoxy-L-rhamnonate hydrolase (EC 3.7.1.-) (characterized) 97% 100% 568.2 2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate isomerase 38% 169.5
L-rhamnose catabolism LRA6 hi 2,4-diketo-3-deoxy-L-rhamnonate hydrolase (EC 3.7.1.-) (characterized) 97% 100% 568.2 2,4-dihydroxy-5-methyl-6-oxohexa-2,4-dienoate isomerase 38% 169.5
D-xylose catabolism HDOP-hydrol med FAA hydrolase family protein (characterized, see rationale) 77% 100% 463 Ureidoglycolate lyase; UGL; Ureidoglycolase; Ureidoglycolatase; Ureidoglycolate hydrolase; EC 4.3.2.3 75% 456.1
L-phenylalanine catabolism fahA lo fumarylacetoacetase (EC 3.7.1.2) (characterized) 37% 70% 142.1 2,4-diketo-3-deoxy-L-rhamnonate hydrolase (EC 3.7.1.-) 97% 568.2
L-tyrosine catabolism fahA lo fumarylacetoacetase (EC 3.7.1.2) (characterized) 37% 70% 142.1 2,4-diketo-3-deoxy-L-rhamnonate hydrolase (EC 3.7.1.-) 97% 568.2
D-xylose catabolism kdaD lo Probable 2-keto-3-deoxyxylonate dehydratase; KDXD; EC 4.2.1.141 (characterized) 32% 58% 65.9 2,4-diketo-3-deoxy-L-rhamnonate hydrolase (EC 3.7.1.-) 97% 568.2

Sequence Analysis Tools

View H281DRAFT_03528 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKLLRYGPKGQEKPGLLDAQGKIRDLSGVVADIDGAALTDEGLAQLRALDPASLPVVDGN
PRMGPCVGKIGKFICIGLNYADHAAESNLPVPAEPVIFNKWTSAISGPNDDVEIPRGSKK
TDWEVELGVVIGKPAKYVDEANALDYVAGYCVINDVSEREWQIERGGTWDKGKGFDTFGP
IGPWMVTRDEVADPQNLSLWLEVDGRRYQNGSTKTMVFGVAKLVSYVSQCMSLQPGDVIS
TGTPPGVGMGVKPNPVYLKPGQTIRLGIEGLGEQTQKTYAAE

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory