Protein H281DRAFT_05513 in Paraburkholderia bryophila 376MFSha3.1
Annotation: FitnessBrowser__Burk376:H281DRAFT_05513
Length: 597 amino acids
Source: Burk376 in FitnessBrowser
Candidate for 27 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-isoleucine catabolism | livG | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-leucine catabolism | livG | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-phenylalanine catabolism | livG | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-proline catabolism | HSERO_RS00895 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-serine catabolism | Ac3H11_1693 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-tyrosine catabolism | Ac3H11_1693 | med | ABC transporter ATP-binding protein (characterized, see rationale) | 41% | 95% | 195.3 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-valine catabolism | livG | med | ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) | 41% | 98% | 188 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-alanine catabolism | braF | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-histidine catabolism | natA | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-leucine catabolism | natA | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-proline catabolism | natA | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-serine catabolism | braF | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-threonine catabolism | braF | med | NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized) | 40% | 93% | 174.9 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-isoleucine catabolism | natA | lo | NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 39% | 95% | 171.4 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-valine catabolism | natA | lo | NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized) | 39% | 95% | 171.4 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| D-alanine catabolism | AZOBR_RS08245 | lo | Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) | 37% | 88% | 159.5 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-proline catabolism | AZOBR_RS08245 | lo | Leucine/isoleucine/valine ABC transporter,ATPase component; EC 3.6.3.- (characterized, see rationale) | 37% | 88% | 159.5 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-arginine catabolism | braF | lo | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 37% | 90% | 154.8 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-glutamate catabolism | braF | lo | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 37% | 90% | 154.8 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-histidine catabolism | braF | lo | ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale) | 37% | 90% | 154.8 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-isoleucine catabolism | livM | lo | High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M (characterized) | 31% | 80% | 146.7 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-leucine catabolism | livM | lo | High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M (characterized) | 31% | 80% | 146.7 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-phenylalanine catabolism | livM | lo | High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M (characterized) | 31% | 80% | 146.7 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-valine catabolism | livM | lo | High-affinity branched-chain amino acid transport system permease protein LivM; LIV-I protein M (characterized) | 31% | 80% | 146.7 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-arginine catabolism | braE | lo | Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) | 32% | 65% | 136 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-glutamate catabolism | braE | lo | Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) | 32% | 65% | 136 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
| L-histidine catabolism | braE | lo | Transmembrane component of a broad range amino acid ABC transporter (characterized, see rationale) | 32% | 65% | 136 | ABC amino acid transporter, ATP-binding, component of Uptake transporter, CamABCD of cholate (steroid) metabolites, 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog | 35% | 309.7 |
Sequence Analysis Tools
View H281DRAFT_05513 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MSAEQNALGLRRARKAPYFLCAILLAGLPWIWSGGFGIHIAQSFCYTAIAVIGVNLLVGL
SGQMSLGQAAFYAIGAYASVLTSLKLGWPIAASVLFGVAVAAAVGTVVGVFALRTRGLYL
AMTTLAVGFVVSILAQRWVGLTGGTMGLSGVPQVDFGDTIHGEIWYLYVVGGTLLVVQIL
NDYVFDSRIGRCLLATRDSEAFAASVGIKVPIVRSAIFAVSAALAGLSGGLFAHQSGFVG
SDAFSVTLSLSLLIAAVIGGLGARIGPLAGTAILMTIVELVAGLDRYGLMVYGGIMLLVL
LVFPQGAAGIVDIWRKPKSARKGSPSPENRSEAIARVDLQLDGCSLTVESVSKQYGGVLA
VSDVSFSVAAGQIHGLIGPNGAGKSTLVNLIAGNYLCDSGRVRLDGADVSNLVTADRAKR
GLARTFQNLQLVEALPVLENVLLGMSSADGHVANFAKWWMGRAFDLPERREALEILAFFG
IEHLCQAYPTELSYGHRKLVELARAIAQRPRLMLLDEPIAGLNGAEAMEVAKVVGRLRDA
GVTILLIEHNMEFVMSLCDSISVLEQGRLIGTGTPEEIRSDERILRAYLGTDEAMKC
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory