Align Na+/solute symporter (characterized, see rationale)
to candidate H281DRAFT_03156 H281DRAFT_03156 solute:Na+ symporter, SSS family
Query= uniprot:B2T7V3 (493 letters) >FitnessBrowser__Burk376:H281DRAFT_03156 Length = 493 Score = 927 bits (2396), Expect = 0.0 Identities = 469/493 (95%), Positives = 480/493 (97%) Query: 1 MSDVNPVNPVAMTVFIAFFVLVTVIGFFAARWKRGDLTQLHEWGLGGRQFGTVISWFLVG 60 M+D+NPVNPVAMTVFIAFFVLVTVIGFFAARWKRGDLTQLHEWGLGGRQFGTVISWFLVG Sbjct: 1 MNDLNPVNPVAMTVFIAFFVLVTVIGFFAARWKRGDLTQLHEWGLGGRQFGTVISWFLVG 60 Query: 61 GDFYTAYTVIAVPALVYSVGAYGFFALPYTIIVYPFVFAVMPKLWKIAHAKNHITAADYV 120 GDFYTAYTVIAVPALVYSVGAYGFFALPYTIIVYPFVFAVMPKLWK+AHAKNHITAADYV Sbjct: 61 GDFYTAYTVIAVPALVYSVGAYGFFALPYTIIVYPFVFAVMPKLWKVAHAKNHITAADYV 120 Query: 121 QGEYGGKWFPAAVAITGIVATMPYIALQLVGMQVVIKGLGVTGEMPLIVAFVILALYTYA 180 GEYGGKWFPAAVA+TGIVATMPYIALQLVGMQVVIKGLGVTGE+PLIVAFVILALYTYA Sbjct: 121 HGEYGGKWFPAAVALTGIVATMPYIALQLVGMQVVIKGLGVTGELPLIVAFVILALYTYA 180 Query: 181 SGLRAPAMIAFVKDIMIYIVVIAAVWLIPAKLGGYAHVFDAADTYFKAKGGATGIILKPT 240 SGLRAPAMIAFVKDIMIYIVVIAAVWLIPAKLGGYAHVFDAADTYFKAKGGATGIILKPT Sbjct: 181 SGLRAPAMIAFVKDIMIYIVVIAAVWLIPAKLGGYAHVFDAADTYFKAKGGATGIILKPT 240 Query: 241 QFTAYASLALGSALAAFMYPHTMTAVLSSSSANTVRKNAIFLPAYTLLLGLIALLGYMAI 300 QFTAYASLALGSALAAFMYPHTMTAVLSSSSA TVRKNAIFLPAYTLLLGLIALLGYMAI Sbjct: 241 QFTAYASLALGSALAAFMYPHTMTAVLSSSSATTVRKNAIFLPAYTLLLGLIALLGYMAI 300 Query: 301 AAGVHVKSASDMVPALFNTLFPSWFVGFAAAAIAISALVPAAIMSIGAANLFTRNLWRPL 360 AAGVHVKSASD+VP LF TLFPSWFVGFAAAAIAISALVPAAIMSIGAANLFTRNLWRPL Sbjct: 301 AAGVHVKSASDIVPTLFGTLFPSWFVGFAAAAIAISALVPAAIMSIGAANLFTRNLWRPL 360 Query: 361 VSPNISPEGEASTAKIVSLVVKFGALLFIVFLPTQYAIDLQLLGGVWILQIFPAIVFSLY 420 VSPNI+PE EAS AKIVSLVVKFGALLFIVFLPTQYAIDLQLLGGVWILQIFPAIVFSLY Sbjct: 361 VSPNITPEAEASNAKIVSLVVKFGALLFIVFLPTQYAIDLQLLGGVWILQIFPAIVFSLY 420 Query: 421 TRRLNTPGLFLGWLAGIVLGTGLAISQGLKPVFALHLGDAVYPVYIGLIALTVNIVVSFV 480 TRRLNTPGLF GWL GIVLGT LAISQGLKPVFALHLGDAVYP+YIGLIAL VNIVV+FV Sbjct: 421 TRRLNTPGLFAGWLVGIVLGTALAISQGLKPVFALHLGDAVYPIYIGLIALVVNIVVTFV 480 Query: 481 VSVLSPRRVVAAA 493 VS +SPRRV +A Sbjct: 481 VSAVSPRRVAVSA 493 Lambda K H 0.328 0.141 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1029 Number of extensions: 42 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 493 Length of database: 493 Length adjustment: 34 Effective length of query: 459 Effective length of database: 459 Effective search space: 210681 Effective search space used: 210681 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory