Align protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) (characterized)
to candidate H281DRAFT_01976 H281DRAFT_01976 PTS system N-acetylglucosamine-specific IIB component, Glc family /PTS system N-acetylglucosamine-specific IIC component, Glc family
Query= BRENDA::P45604 (651 letters) >FitnessBrowser__Burk376:H281DRAFT_01976 Length = 599 Score = 543 bits (1400), Expect = e-159 Identities = 279/497 (56%), Positives = 353/497 (71%), Gaps = 21/497 (4%) Query: 8 QRLGRALQLPIAVLPVAALLLRFGQPDLLNVPFIAQAGGAIFDNLALIFAIGVASSWSKD 67 QRLGRAL LPIAVLPVA LLLR GQPD+ N+ IA AGGAIFDNL L+FAIGVA ++KD Sbjct: 10 QRLGRALMLPIAVLPVAGLLLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKD 69 Query: 68 NAGSAALAGAVGYFVMTKAMVTINPEINMGVLAGIITGLVAGAVYNRWAGIKLPDFLSFF 127 N G A LAGA+GY + M IN ++NMGVL+GI+ G+VAG +YNR+ IKLPD+L+FF Sbjct: 70 NNGVAGLAGAIGYLIEVAVMKDINDKLNMGVLSGIVAGIVAGLLYNRYKDIKLPDYLAFF 129 Query: 128 GGKRFVPIATGFFCLILAAIFGYVWPPVQHAIHSGGEWIVSAGALGSGIFGFINRLLIPT 187 GGKRFVPI TG CL L +FGYVW PVQ I + G W+ +AGALG+ +FG +NRLL+ T Sbjct: 130 GGKRFVPIVTGVVCLALGILFGYVWQPVQSVIDTAGHWLTTAGALGAFVFGVLNRLLLVT 189 Query: 188 GLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFGLPGAALA 247 GLH +LN++ WF G FT G GD++RF+AGD TAG FM+GFFP+MMFGLP A LA Sbjct: 190 GLHHILNSLTWFVFGTFTPPGGAAVTGDLHRFFAGDPTAGTFMTGFFPVMMFGLPAACLA 249 Query: 248 MYLAAPKARRPMVGGMLLSVAITAFLTGVTEPLEFLFLFLAPLLYLLHAVLTGISLFIAT 307 M+ APK RR +VGG+L S+A+T+FLTGVTEP+EF F+FLAP+LY++HA+LTG++L I + Sbjct: 250 MFHEAPKERRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHALLTGLALAICS 309 Query: 308 ALGIHAGFSFSAGAIDYVLMYSLPAASKNVWMLLVMGVVFFFVYFLLFSAVIRMFNLKTP 367 ALGIH GF+FSAGAIDYVL Y L S W+ + +G+V+ VY+ LF IR FN+ TP Sbjct: 310 ALGIHLGFTFSAGAIDYVLNYGL---STKGWLAIPVGIVYAVVYYGLFRFFIRKFNMATP 366 Query: 368 GREDKAADVVTEEANSNTEEGLTQ--------LATSYIAAVGGTDNLKAIDACITRLRLT 419 GRE AAD E+ +S G A YIAA+GG NL +DAC TRLRL+ Sbjct: 367 GREPAAAD---EQVDSFAAGGFVSPVAGAAVPRAQRYIAALGGAANLSVVDACTTRLRLS 423 Query: 420 VGDSAKVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRG-------PVA 472 V DS KV++ K +GA GV+K +QVI+G +A+ I DE++ + RG A Sbjct: 424 VVDSGKVSENELKTIGARGVLKRGATNVQVIIGPEADIIADEIRTAMERGGNDAGVVKPA 483 Query: 473 AAAAAPAGNVATAAPAA 489 A+AAAPA +AAP+A Sbjct: 484 ASAAAPAAVSTSAAPSA 500 Score = 37.0 bits (84), Expect = 3e-06 Identities = 33/112 (29%), Positives = 50/112 (44%), Gaps = 15/112 (13%) Query: 374 ADVVTEEANSNTEEGLTQLATS--------YIAAVGGTDNLKAIDA-CITRLRLTVGDSA 424 A V T A S T G + A ++A GG N+K++DA TRLR+ V D + Sbjct: 490 AAVSTSAAPSATVGGASAAANGPLDPDPLRWLAVFGGAGNVKSLDAVAATRLRIVVQDPS 549 Query: 425 KVNDAACKRLGASGVVKLNKQTIQVIVGAKAESIGDEMKKVVTRGPVAAAAA 476 V+ +RL + T+ ++VG A + + TR P + AA Sbjct: 550 AVDR---QRLATLDTAWVAADTVHIVVGDAARRYA---QALATRMPASGGAA 595 Lambda K H 0.323 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1036 Number of extensions: 65 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 2 Length of query: 651 Length of database: 599 Length adjustment: 37 Effective length of query: 614 Effective length of database: 562 Effective search space: 345068 Effective search space used: 345068 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory